connexin 47 Antibody (391CT6.4.3): sc-517325

Connexin 47 Antibody (391CT6.4.3) is a mouse monoclonal IgM antibody that detects connexin 47 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Connexin 47, also known as gap junction protein gamma 2 (GJC2), is a vital transmembrane protein that forms hexameric structures called connexons, which are essential for the formation of gap junctions that facilitate direct intercellular communication. These gap junctions allow the passage of ions and small molecules between adjacent cells, playing a critical role in maintaining cellular homeostasis and coordinating cellular responses. Notably, connexin 47 is predominantly expressed in oligodendrocytes within the central nervous system (CNS), where connexin 47 is crucial for myelination—the process that insulates neuronal axons to ensure rapid nerve impulse conduction. Proper functioning of connexin 47 is essential for CNS integrity, as mutations in the GJC2 gene encoding connexin 47 have been linked to neurological disorders, including Pelizaeus-Merzbacher-like disease type 1, which is characterized by impaired myelination and subsequent neurological deficits. Anti-connexin 47 antibody (391CT6.4.3) is available in a non-conjugated form, making anti-connexin 47 antibody (391CT6.4.3) an invaluable tool for researchers exploring the role of connexin 47 in neural development, myelination processes, and related pathologies, thereby contributing to a deeper understanding of CNS health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

connexin 47 Antibody (391CT6.4.3) References:

1. Connexin 47 (Cx47)-deficient mice with enhanced green fluorescent protein reporter gene reveal predominant oligodendrocytic expression of Cx47 and display vacuolized myelin in the CNS.  |  Odermatt, B., et al. 2003. J Neurosci. 23: 4549-59. PMID: 12805295
2. Unique distributions of the gap junction proteins connexin29, connexin32, and connexin47 in oligodendrocytes.  |  Kleopa, KA., et al. 2004. Glia. 47: 346-57. PMID: 15293232
3. Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.  |  Kamasawa, N., et al. 2005. Neuroscience. 136: 65-86. PMID: 16203097
4. Loss-of-function GJA12/Connexin47 mutations cause Pelizaeus-Merzbacher-like disease.  |  Orthmann-Murphy, JL., et al. 2007. Mol Cell Neurosci. 34: 629-41. PMID: 17344063
5. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of the ERK/Id4 pathway.  |  Liu, Z., et al. 2017. Cell Cycle. 16: 714-722. PMID: 28278052
6. Astrocytes regulate the expression of Sp1R3 on oligodendrocyte progenitor cells through Cx47 and promote their proliferation.  |  Xu, D., et al. 2017. Biochem Biophys Res Commun. 490: 670-675. PMID: 28634078
7. Different Mutations of Gap Junction Connexin 47 Lead to Discrepant Activation of Unfolded Protein Response Pathway in Pelizaeus-Merzbacher-Like Disease.  |  Chen, N., et al. 2017. Neuropediatrics. 48: 426-431. PMID: 28712094
8. Directional coupling of oligodendrocyte connexin-47 and astrocyte connexin-43 gap junctions.  |  Fasciani, I., et al. 2018. Glia. 66: 2340-2352. PMID: 30144323
9. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression.  |  Jiang, L., et al. 2019. Eur Rev Med Pharmacol Sci. 23: 3012-3020. PMID: 31002152
10. Beneficial contribution of induced pluripotent stem cell-progeny to Connexin 47 dynamics during demyelination-remyelination.  |  Mozafari, S., et al. 2021. Glia. 69: 1094-1109. PMID: 33301181
11. Investigating oligodendrocyte connexins: Heteromeric interactions between Cx32 and mutant or wild-type forms of Cx47 do not contribute to or modulate gap junction function.  |  Abrams, CK., et al. 2021. Glia. 69: 1882-1896. PMID: 33835612