connexin 31.9 Double Nickase Plasmid (m2): sc-436134-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 31.9 Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- connexin 31.9 Double Nickase Plasmid (m2) and connexin 31.9 Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Gjd3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 31.9 Double Nickase Plasmid (m2) | sc-436134-NIC-2 | 20 µg | $410.00 |
Mouse Gjd3 encodes connexin 31.9, a gap junction protein that assembles into connexons to mediate direct intercellular communication via exchange of ions and small metabolites. Connexin-dependent coupling contributes to tissue homeostasis by coordinating electrical and metabolic signaling, influencing processes such as proliferation, differentiation, and stress responses. Altered connexin expression or channel function is associated with dysregulated cell–cell signaling and has been investigated in contexts including epithelial barrier integrity, inflammation, and tumor biology, making Gjd3 a useful target for probing gap junction pathway contributions to physiology and disease-relevant phenotypes.
connexin 31.9 Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Gjd3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gjd3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gjd3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gjd3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.