Complexin-3 Antibody (C-8): sc-365941
- Complexin-3 Antibody (C-8) is a mouse monoclonal IgG2a κ Complexin-3 antibody provided at 200 µg/ml
- raised against amino acids 1-44 mapping at the N-terminus of Complexin-3 of human origin
- Complexin-3 Antibody (C-8) is recommended for detection of Complexin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Complexin-3 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Complexin-3 Antibody (C-8). This work is in progress.
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Complexin-3 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Complexin-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Complexin-3 antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Complexin-3, also referred to as CPXIII, CPX-III, Nbla11589, or CPLX3, is a 158 amino acid protein that plays a crucial role in regulating synaptic vesicle exocytosis, which is essential for neurotransmitter release in the nervous system. Complexin-3 is primarily located in presynaptic terminals of neurons, where Complexin-3 binds to the SNARE core complex, which includes SNAP-25, VAMP-2, and Syntaxin 1. Complexin-3 at these sites is vital for proper functioning of synaptic transmission, as Complexin-3 enhances the efficiency of neurotransmitter release by stabilizing the SNARE complex during the calcium-triggering step of exocytosis. Neurons deficient in Complexin-3 exhibit impaired neurotransmitter release, highlighting Complexin-3′s importance in maintaining synaptic communication and overall neuronal health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Complexin-3 Antibody (C-8) References:
- Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions. | Pabst, S., et al. 2000. J Biol Chem. 275: 19808-18. PMID: 10777504
- Hippocampal synaptic pathology in schizophrenia, bipolar disorder and major depression: a study of complexin mRNAs. | Eastwood, SL. and Harrison, PJ. 2000. Mol Psychiatry. 5: 425-32. PMID: 10889554
- Involvement of complexin II in synaptic plasticity in the CA1 region of the hippocampus: the use of complexin II-lacking mice. | Huang, GZ., et al. 2000. Jpn J Pharmacol. 84: 179-87. PMID: 11128041
- Complexins regulate a late step in Ca2+-dependent neurotransmitter release. | Reim, K., et al. 2001. Cell. 104: 71-81. PMID: 11163241
- SNARE complex oligomerization by synaphin/complexin is essential for synaptic vesicle exocytosis. | Tokumaru, H., et al. 2001. Cell. 104: 421-32. PMID: 11239399
- Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system. | Xue, M., et al. 2008. Proc Natl Acad Sci U S A. 105: 7875-80. PMID: 18505837
- Complexin and Ca2+ stimulate SNARE-mediated membrane fusion. | Yoon, TY., et al. 2008. Nat Struct Mol Biol. 15: 707-13. PMID: 18552825
- Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4. | Reim, K., et al. 2009. J Cell Sci. 122: 1352-61. PMID: 19386896
- Complexins: cytosolic proteins that regulate SNAP receptor function. | McMahon, HT., et al. 1995. Cell. 83: 111-9. PMID: 7553862
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Complexin-3 Antibody (C-8) | sc-365941 | 200 µg/ml | $322.00 | |||
Complexin-3 Antibody (C-8) AC | sc-365941 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Complexin-3 Antibody (C-8) HRP | sc-365941 HRP | 200 µg/ml | $322.00 | |||
Complexin-3 Antibody (C-8) FITC | sc-365941 FITC | 200 µg/ml | $336.00 | |||
Complexin-3 Antibody (C-8) PE | sc-365941 PE | 200 µg/ml | $349.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 488 | sc-365941 AF488 | 200 µg/ml | $364.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 546 | sc-365941 AF546 | 200 µg/ml | $364.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 594 | sc-365941 AF594 | 200 µg/ml | $364.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 647 | sc-365941 AF647 | 200 µg/ml | $364.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 680 | sc-365941 AF680 | 200 µg/ml | $364.00 | |||
Complexin-3 Antibody (C-8) Alexa Fluor® 790 | sc-365941 AF790 | 200 µg/ml | $364.00 |