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Collagenase Type II (CAS 9001-12-1)

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Alternate Names:
Clostridiopeptidase A
Application:
CAS: 9001-12-1.
CAS Number:
9001-12-1
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Collagenase Type II is a specific form of collagenase, an enzyme that catalyzes the breakdown of collagen, the most abundant protein in the animal kingdom and a key component of connective tissues. This particular type of collagenase has a defined substrate specificity and is used extensively in various research fields, especially in tissue engineering, cell biology, and biochemistry. The primary role of Collagenase Type II in research is to facilitate the dissociation of tissues into single cells or smaller aggregates, making it indispensable in the process of isolating cells from tissues, such as in primary cell culture preparations. This enzymatic action is for studies that require a detailed analysis of cells in their isolated state, such as in stem cell research, cancer biology, and immunology. The ability of Collagenase Type II to effectively and gently dissociate cells while preserving their viability and functionality is key to its widespread use in these fields.


Collagenase Type II (CAS 9001-12-1) References

  1. Analysis of collagenase-cleavage of type II collagen using a neoepitope ELISA.  |  Downs, JT., et al. 2001. J Immunol Methods. 247: 25-34. PMID: 11150534
  2. Matrix metalloproteinase-9 facilitates remyelination in part by processing the inhibitory NG2 proteoglycan.  |  Larsen, PH., et al. 2003. J Neurosci. 23: 11127-35. PMID: 14657171
  3. Contact dermatitis with clostridiopeptidase A contained in Noruxol ointment.  |  Foti, C., et al. 2007. Contact Dermatitis. 56: 361-2. PMID: 17577383
  4. Collagenolysis of rat tail tendons by crude corneal collagenase and clostridiopeptidase A.  |  Prause, JU. 1976. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 199: 249-54. PMID: 180847
  5. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone.  |  Zhu, H., et al. 2010. Nat Protoc. 5: 550-60. PMID: 20203670
  6. Enzyme-accelerated hydrolysis of polyglycolic acid.  |  Williams, DF. and Mort, E. 1977. J Bioeng. 1: 231-8. PMID: 210160
  7. Quantitative analysis of denatured collagen by collagenase digestion and subsequent MALDI-TOF mass spectrometry.  |  Nimptsch, A., et al. 2011. Cell Tissue Res. 343: 605-17. PMID: 21274570
  8. Anti-proteolytic property and bonding durability of mussel adhesive protein-modified dentin adhesive interface.  |  Fang, H., et al. 2017. Dent Mater. 33: 1075-1083. PMID: 28754254
  9. A rabbit model of corneal Ectasia generated by treatment with collagenase type II.  |  Qiao, J., et al. 2018. BMC Ophthalmol. 18: 94. PMID: 29653563
  10. Studies on the stimulation of the bacterial, collagenolytic enzyme clostridiopeptidase A by cobalt (II) ions.  |  Evans, CH. and Mason, GC. 1986. Int J Biochem. 18: 89-92. PMID: 3002884
  11. Allergic contact dermatitis to clostridiopeptidase A with a nummular eczema-like spread.  |  Foti, C., et al. 2022. Contact Dermatitis. 86: 122-123. PMID: 34590321
  12. Animal model of corneal ectasia in rabbits by intrastromal injection of type II collagenase.  |  Cano-Gómez, LE., et al. 2023. Arch Soc Esp Oftalmol (Engl Ed). 98: 206-212. PMID: 36871853
  13. Biochemical properties of anti-inflammatory drugs. VII. Inhibition of proteolytic enzymes in connective tissue by chloroquine (resochin) and related antimalarial antirheumatic drugs.  |  Cowey, FK. and Whitehouse, MW. 1966. Biochem Pharmacol. 15: 1071-84. PMID: 4292639

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Collagenase Type II, 25 mg

sc-506177C
25 mg
$84.00

Collagenase Type II, 100 mg

sc-506177
100 mg
$130.00

Collagenase Type II, 500 mg

sc-506177A
500 mg
$354.00

Collagenase Type II, 1 g

sc-506177D
1 g
$580.00

Collagenase Type II, 5 g

sc-506177B
5 g
$2326.00