COL8A2 Antibody (1F4): sc-293350

COL8A2 Antibody (1F4) is a mouse monoclonal IgG2a kappa light chain antibody that detects COL8A2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-COL8A2 antibody (1F4) is available as a non-conjugated monoclonal isotype antibody. COL8A2 protein, also known as collagen alpha-2(VIII) chain or endothelial collagen, is a crucial 703 amino acid secreted protein that plays a significant role in maintaining the structural integrity of the Descemet membrane, which is essential for corneal endothelial cell function. This membrane acts as a barrier and is vital for regulating fluid balance in the cornea, thereby contributing to transparency and proper vision. COL8A2′s ability to form both homodimers and heterodimers with COL8A1 is important for structural properties and functionality within the extracellular matrix. Defects in COL8A2 are linked to several corneal dystrophies, including posterior polymorphous corneal dystrophy and Fuchs endothelial corneal dystrophy, both of which can lead to visual impairment in adulthood. Additionally, mutations in COL8A2 are responsible for posterior polymorphous corneal dystrophy 2, a rare familial disorder that manifests from birth.

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COL8A2 Antibody (1F4) References:

1. Missense mutations in COL8A2, the gene encoding the alpha2 chain of type VIII collagen, cause two forms of corneal endothelial dystrophy.  |  Biswas, S., et al. 2001. Hum Mol Genet. 10: 2415-23. PMID: 11689488
2. Inheritance of a novel COL8A2 mutation defines a distinct early-onset subtype of fuchs corneal dystrophy.  |  Gottsch, JD., et al. 2005. Invest Ophthalmol Vis Sci. 46: 1934-9. PMID: 15914606
3. Migration and growth are attenuated in vascular smooth muscle cells with type VIII collagen-null alleles.  |  Adiguzel, E., et al. 2006. Arterioscler Thromb Vasc Biol. 26: 56-61. PMID: 16269661
4. H244R VSX1 is associated with selective cone ON bipolar cell dysfunction and macular degeneration in a PPCD family.  |  Valleix, S., et al. 2006. Invest Ophthalmol Vis Sci. 47: 48-54. PMID: 16384943
5. Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.  |  Turner, NJ., et al. 2006. Circulation. 114: 820-9. PMID: 16908762
6. No pathogenic mutations identified in the COL8A1 and COL8A2 genes in familial Fuchs corneal dystrophy.  |  Aldave, AJ., et al. 2006. Invest Ophthalmol Vis Sci. 47: 3787-90. PMID: 16936088
7. The alpha 2(VIII) collagen gene. A novel member of the short chain collagen family located on the human chromosome 1.  |  Muragaki, Y., et al. 1991. J Biol Chem. 266: 7721-7. PMID: 2019595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COL8A2 Antibody (1F4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-COL8A2 Antibody (1F4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COL8A2 Antibody (1F4) Specifications

Alternate Product Name: COL8A2

Product Clone Name: 1F4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 626-696

Epitope Target: COL8A2

Epitope Species: human

Product Reactivity/Detection: Collagen α2 Type VIII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for COL8A2 Antibody (1F4): N/A