Molecular structure of Coelenterazine-h, CAS Number: 50909-86-9
Coelenterazine-h (CAS 50909-86-9)
Alternate Names:
2-Deoxycoelenterazine; CLZ-h; 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
Application:
Coelenterazine-h is an intracellular luminophore for measuring superoxide and Ca2+ in cells transfected with apoaequorin cDNA
CAS Number:
50909-86-9
Purity:
>95%
Molecular Weight:
407.46
Molecular Formula:
C26H21N3O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
QUICK LINKS
Ordering Information
Description
Technical Information
Safety Information
SDS & Certificate of Analysis
Coelenterazine-h, Synthetic is a highly sensitive and a specific intracellular luminophore that is useful for measuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA. Coelenterazine-h, Synthetic exhibits nearly 16-fold greater luminescence intensity (emission maximum ~466 nm half-total time of 0.6 - 1.2 sec) as compared to the native Coelenterazine. It is known for its significantly higher luminescence intensity compared to its native counterpart, making it a preferred choice for various biochemical applications. This property enhances its utility in research, particularly in studies involving luminescence-based assays.
Coelenterazine-h (CAS 50909-86-9) References
- The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla Luciferase. | Herbst, KJ., et al. 2009. PLoS One. 4: e5642. PMID: 19461967
- General Bioluminescence Resonance Energy Transfer Homogeneous Immunoassay for Small Molecules Based on Quantum Dots. | Yu, X., et al. 2016. Anal Chem. 88: 3512-20. PMID: 26948147
- Semi-synthetic aequorins with improved sensitivity to Ca2+ ions. | Shimomura, O., et al. 1989. Biochem J. 261: 913-20. PMID: 2803250
- Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells. | Harikumar, KG., et al. 2017. Bio Protoc. 7: PMID: 29423426
- Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody-Nanoluciferase Fusion. | Wang, F., et al. 2020. Anal Chem. 92: 11935-11942. PMID: 32702970
- Self-luminescent photodynamic therapy using breast cancer targeted proteins. | Kim, EH., et al. 2020. Sci Adv. 6: PMID: 32917700
- Preparation of Luciferase-fused Peptides for Immunoassay of Amyloid Beta. | Sakono, M., et al. 2021. Anal Sci. 37: 759-763. PMID: 33583860
- The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogues. | Shimomura, O., et al. 1993. Biochem J. 296 (Pt 3): 549-51. PMID: 8280050
- Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. | Knight, MR., et al. 1993. J Cell Biol. 121: 83-90. PMID: 8458875
- Light-emitting properties of recombinant semi-synthetic aequorins and recombinant fluorescein-conjugated aequorin for measuring cellular calcium. | Shimomura, O., et al. 1993. Cell Calcium. 14: 373-8. PMID: 8519061
- Regeneration and luminescence of aequorin in Chinese hamster ovary cells transformed with cDNA for apoaequorin. | Sanchez-Bueno, A., et al. 1996. Int J Biochem Cell Biol. 28: 1045-9. PMID: 8930127
- Membrane permeability of coelenterazine analogues measured with fish eggs. | Shimomura, O. 1997. Biochem J. 326 (Pt 2): 297-8. PMID: 9291095
- Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx. | Nebl, T. and Fisher, PR. 1997. J Cell Sci. 110 (Pt 22): 2845-53. PMID: 9427292
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Coelenterazine-h, 50 µg | sc-205908 | 50 µg | $176.00 |