CNOT2 Antibody (2191C2a): sc-81229

CNOT2 Antibody (2191C2a) is a mouse monoclonal IgG2a antibody that detects CNOT2 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-CNOT2 antibody (2191C2a) is available as a non-conjugated monoclonal isotype antibody. CNOT2, or CCR4-NOT transcription complex subunit 2, is a crucial component of the CCR4-NOT complex, which plays a significant role in regulating gene expression and mRNA degradation. CNOT2 is primarily located in the cytoplasm and nucleus, where CNOT2 participates in the post-transcriptional regulation of mRNA stability and translation. CNOT2 localization allows interaction with various mRNA molecules and other regulatory proteins, thereby influencing cellular responses to environmental changes and developmental cues. CNOT2 interacts with other subunits of the CCR4-NOT complex, such as CNOT1 and CNOT3, which further enhances regulatory capabilities. CCR4-NOT complex involvement in transcriptional repression, particularly through RNA polymerase II action, underscores CNOT2′s importance in maintaining proper gene expression levels, essential for normal cellular function and development.

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CNOT2 Antibody (2191C2a) References:

1. Repression of promoter activity by CNOT2, a subunit of the transcription regulatory Ccr4-not complex.  |  Zwartjes, CG., et al. 2004. J Biol Chem. 279: 10848-54. PMID: 14707134
2. The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution.  |  Lenssen, E., et al. 2005. Mol Cell Biol. 25: 488-98. PMID: 15601868
3. The cyclin-dependent kinase 11 interacts with NOT2.  |  Shi, J. and Nelson, MA. 2005. Biochem Biophys Res Commun. 334: 1310-6. PMID: 16039607
4. Involvement of the SMRT/NCoR-HDAC3 complex in transcriptional repression by the CNOT2 subunit of the human Ccr4-Not complex.  |  Jayne, S., et al. 2006. Biochem J. 398: 461-7. PMID: 16712523
5. Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription.  |  Winkler, GS., et al. 2006. EMBO J. 25: 3089-99. PMID: 16778766
6. CCR4/NOT complex associates with the proteasome and regulates histone methylation.  |  Laribee, RN., et al. 2007. Proc Natl Acad Sci U S A. 104: 5836-41. PMID: 17389396
7. Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1.  |  Garapaty, S., et al. 2008. J Biol Chem. 283: 6806-16. PMID: 18180299
8. CNOT2 promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231 breast cancer cells.  |  Sohn, EJ., et al. 2018. Cancer Lett. 412: 88-98. PMID: 29024811
9. Delineation of the clinical profile of CNOT2 haploinsufficiency and overview of the IDNADFS phenotype.  |  Niceta, M., et al. 2023. Clin Genet. 103: 156-166. PMID: 36224108
10. Differential activation of the clustered homeobox genes CNOT2 and CNOT1 during notogenesis in the chick.  |  Stein, S., et al. 1996. Dev Biol. 180: 519-33. PMID: 8954724
11. Gastrulation and homeobox genes in chick embryos.  |  Lemaire, L. and Kessel, M. 1997. Mech Dev. 67: 3-16. PMID: 9347911

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CNOT2 Antibody (2191C2a) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CNOT2 Antibody (2191C2a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CNOT2 Antibody (2191C2a) Specifications

Alternate Product Name: CNOT2

Product Clone Name: 2191C2a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: CNOT2

Epitope Species: human

Product Reactivity/Detection: CNOT2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CNOT2 Antibody (2191C2a): N/A