CNF1 Antibody (NG8): sc-52655

CNF1 Antibody (NG8) is a mouse monoclonal IgG2a antibody that detects the CNF1 protein of E. coli origin by western blotting (WB). Anti-CNF1 antibody (NG8) is available as the non-conjugated format. Cytotoxic necrotizing factor 1 (CNF1) is a potent toxin produced by uropathogenic and meningitis-causing strains of Escherichia coli, and CNF1′s role in pathogenesis is critical due to its ability to permanently activate Rho-GTP-binding proteins, including Rho, Rac, and Cdc42, through deamidation or transglutamination. This activation leads to significant reorganization of the actin cytoskeleton, resulting in the formation of large stress fibers and increased focal adhesion points, which are essential for cell motility and stability. CNF1 internalization into host cells occurs via endocytosis, followed by transport into late endosomes, where CNF1′s middle domain, characterized by two hydrophobic helices, facilitates translocation across the membrane upon acidification. CNF1′s action extends to potential interference with mitotic processes, contributing to aneuploidy and multinucleation, which are hallmarks of various pathological conditions. CNF1 (NG8) monoclonal antibody enables valuable insights into bacterial pathogenesis mechanisms and cellular responses to this unique toxin.

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CNF1 Antibody (NG8) References:

1. Effects of cytotoxic necrotizing factor 1 and lethal toxin on actin cytoskeleton and VE-cadherin localization in human endothelial cell monolayers.  |  Vouret-Craviari, V., et al. 1999. Infect Immun. 67: 3002-8. PMID: 10338511
2. The cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli.  |  Boquet, P. 2001. Toxicon. 39: 1673-80. PMID: 11595630
3. E. coli CNF1 toxin: a two-in-one system for host-cell invasion.  |  Landraud, L., et al. 2004. Int J Med Microbiol. 293: 513-8. PMID: 15149026
4. Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin.  |  Doye, A., et al. 2006. Methods Enzymol. 406: 447-56. PMID: 16472677
5. CNF1-induced ubiquitylation and proteasome destruction of activated RhoA is impaired in Smurf1-/- cells.  |  Boyer, L., et al. 2006. Mol Biol Cell. 17: 2489-97. PMID: 16540523
6. Is the Rac GTPase-activating toxin CNF1 a smart hijacker of host cell fate?  |  Malorni, W. and Fiorentini, C. 2006. FASEB J. 20: 606-9. PMID: 16581968
7. Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1.  |  Schmidt, G., et al. 1997. Nature. 387: 725-9. PMID: 9192900
8. Cytotoxic necrotizing factor 1 from Escherichia coli: a toxin with a new intracellular activity for eukaryotic cells.  |  Boquet, P. 1998. Folia Microbiol (Praha). 43: 285-9. PMID: 9717256
9. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli.  |  Meysick, KC., et al. 2001. Infect Immun. 69: 2066-74. PMID: 11254559

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CNF1 Antibody (NG8) has 5 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CNF1 Antibody (NG8) Specifications

Alternate Product Name: CNF1

Product Clone Name: NG8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 704-730

Epitope Target: CNF1

Epitope Species: E. coli

Product Reactivity/Detection: CNF1

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CNF1 Antibody (NG8): N/A