CNF1/2 Antibody (JC4): sc-52656
- CNF1/2 Antibody (JC4) is a mouse monoclonal IgG2a CNF1/2 antibody provided at 100 µg/ml
- raised against the N-terminal binding domain of CNF1/2, corresponding to amino acids 169-191 of E. coli origin
- recommended for detection of CNF1 and CNF2 of E. coli origin by WB
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CNF1/2 Antibody (JC4). This work is in progress.
CNF1/2 Antibody (JC4) is a mouse monoclonal IgG2a antibody that detects CNF1/2 protein of E. coli origin by western blotting (WB). Anti-CNF1/2 antibody (JC4) is available as the non-conjugated format. Cytotoxic necrotizing factor 1 (CNF1) is a potent toxin produced by uropathogenic and meningitis-causing Escherichia coli, and plays a critical role in manipulating host cell signaling pathways. Specifically, CNF1 catalyzes the permanent activation of Rho-GTP-binding proteins, including Rho, Rac, and Cdc42, through deamidation or transglutamination. This activation leads to significant reorganization of the actin cytoskeleton, resulting in the formation of large stress fibers and an increase in focal adhesion points, which are essential for cell adhesion and migration. CNF1 internalization into cells occurs via endocytosis, followed by transport into late endosomes, where CNF1 exerts effects. Notably, CNF1 may interfere with mitotic catastrophe, a form of cell death linked to abnormal mitosis, ultimately contributing to aneuploidy and multinucleation. Additionally, CNF2, another toxin produced by Escherichia coli, induces cellular death, hyperploidy, and cytoskeletal reorganization in adherent cell lines, further emphasizing the importance of these toxins in bacterial pathogenesis and their potential implications in understanding cellular processes.
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CNF1/2 Antibody (JC4) References:
- Effects of cytotoxic necrotizing factor 1 and lethal toxin on actin cytoskeleton and VE-cadherin localization in human endothelial cell monolayers. | Vouret-Craviari, V., et al. 1999. Infect Immun. 67: 3002-8. PMID: 10338511
- Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. | Meysick, KC., et al. 2001. Infect Immun. 69: 2066-74. PMID: 11254559
- The cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli. | Boquet, P. 2001. Toxicon. 39: 1673-80. PMID: 11595630
- E. coli CNF1 toxin: a two-in-one system for host-cell invasion. | Landraud, L., et al. 2004. Int J Med Microbiol. 293: 513-8. PMID: 15149026
- Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin. | Doye, A., et al. 2006. Methods Enzymol. 406: 447-56. PMID: 16472677
- CNF1-induced ubiquitylation and proteasome destruction of activated RhoA is impaired in Smurf1-/- cells. | Boyer, L., et al. 2006. Mol Biol Cell. 17: 2489-97. PMID: 16540523
- Is the Rac GTPase-activating toxin CNF1 a smart hijacker of host cell fate? | Malorni, W. and Fiorentini, C. 2006. FASEB J. 20: 606-9. PMID: 16581968
- Uncoupling of S-phase and mitosis by recombinant cytotoxic necrotizing factor 2 (CNF2). | Denko, N., et al. 1997. Exp Cell Res. 234: 132-8. PMID: 9223378
- Cytotoxic necrotizing factor 1 from Escherichia coli: a toxin with a new intracellular activity for eukaryotic cells. | Boquet, P. 1998. Folia Microbiol (Praha). 43: 285-9. PMID: 9717256
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNF1/2 Antibody (JC4) | sc-52656 | 100 µg/ml | $322.00 |