CMAH Antibody (E-7): sc-365023

CMAH Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects CMAH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CMAH (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CMAH, or cytidine monophospho-N-acetylneuraminic acid hydroxylase, plays a crucial role in the biosynthesis of sialic acids, specifically catalyzing the conversion of CMP-N-acetylneuraminic acid (Neu5Ac) into its hydroxylated form, CMP-N-glycolylneuraminic acid (Neu5Gc). This enzymatic activity is significant because sialic acids are essential for various biological processes, including cell-cell recognition, immune response modulation, and pathogen interaction, making CMAH a key player in cellular communication and immune evasion. CMAH is expressed in all tissues except the brain and exists in two isoforms due to alternative splicing, with isoform 2 localized in the endoplasmic reticulum. CMAH and its products at the cell surface can influence cellular interactions and signaling pathways, underscoring the importance of this protein in both health and disease.

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CMAH Antibody (E-7) References:

1. Inactivation of CMP-N-acetylneuraminic acid hydroxylase occurred prior to brain expansion during human evolution.  |  Chou, HH., et al. 2002. Proc Natl Acad Sci U S A. 99: 11736-41. PMID: 12192086
2. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation.  |  Naito, Y., et al. 2007. Mol Cell Biol. 27: 3008-22. PMID: 17296732
3. N-glycolylneuraminic acid deficiency in mice: implications for human biology and evolution.  |  Hedlund, M., et al. 2007. Mol Cell Biol. 27: 4340-6. PMID: 17420276
4. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) mutations associated with the domestic cat AB blood group.  |  Bighignoli, B., et al. 2007. BMC Genet. 8: 27. PMID: 17553163
5. Multiple changes in sialic acid biology during human evolution.  |  Varki, A. 2009. Glycoconj J. 26: 231-45. PMID: 18777136
6. The origin of malignant malaria.  |  Rich, SM., et al. 2009. Proc Natl Acad Sci U S A. 106: 14902-7. PMID: 19666593
7. Phylogenetic Distribution of CMP-Neu5Ac Hydroxylase (CMAH), the Enzyme Synthetizing the Proinflammatory Human Xenoantigen Neu5Gc.  |  Peri, S., et al. 2018. Genome Biol Evol. 10: 207-219. PMID: 29206915
8. Genetic knockout of porcine GGTA1 or CMAH/GGTA1 is associated with the emergence of neo-glycans.  |  Morticelli, L., et al. 2023. Xenotransplantation. 30: e12804. PMID: 37148126
9. Cmah deficiency blunts cellular senescence in adipose tissues and improves whole-body glucose metabolism in aged mice.  |  Takeda, R., et al. 2023. Geriatr Gerontol Int. 23: 958-964. PMID: 37968438
10. Inactivation of the CMAH gene and deficiency of Neu5Gc play a role in human brain evolution.  |  Liu, Y., et al. 2025. Inflamm Regen. 45: 5. PMID: 39920734
11. Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid.  |  Kawano, T., et al. 1995. J Biol Chem. 270: 16458-63. PMID: 7608218
12. A structural difference between the cell surfaces of humans and the great apes.  |  Muchmore, EA., et al. 1998. Am J Phys Anthropol. 107: 187-98. PMID: 9786333

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CMAH Antibody (E-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CMAH Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CMAH Antibody (E-7) Specifications

Alternate Product Name: CMAH

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 3-242

Epitope Target: CMAH

Epitope Species: mouse

Product Reactivity/Detection: CMAH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CMAH Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin