Clostridium difficile Toxin A Antibody (PCG4): sc-57685

Clostridium difficile Toxin A Antibody (PCG4) is a mouse monoclonal IgG2a antibody that detects Clostridium difficile Toxin A protein by enzyme-linked immunosorbent assay (ELISA). Anti-Clostridium difficile Toxin A antibody (PCG4) is available as a non-conjugated format. Clostridium difficile Toxin A plays a critical role in the pathogenicity of Clostridium difficile, a major nosocomial pathogen responsible for antibiotic-associated colitis. This toxin disrupts intestinal epithelial cells, leading to severe inflammatory diarrhea. Structurally, Clostridium difficile Toxin A comprises multiple functional domains, including a receptor-binding domain that facilitates attachment to intestinal epithelial cell surface carbohydrates, and an enzymatic domain exhibiting glucosyltransferase activity. This unique structure enables modification of Rho family GTPases, which are crucial for maintaining cytoskeleton integrity and cell signaling pathways. Disruption of these pathways results in significant apoptosis of colonocytes, contributing to ulcer formation and pseudomembranes through activation of p38 MAPK, p53, and caspase-3. The ability of Clostridium difficile Toxin A to induce cellular damage underscores its importance in pathogenesis, making Clostridium difficile Toxin A (PCG4) antibody valuable for research and diagnostic applications.

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Clostridium difficile Toxin A Antibody (PCG4) References:

1. Corticotropin-releasing hormone (CRH) requirement in Clostridium difficile toxin A-mediated intestinal inflammation.  |  Anton, PM., et al. 2004. Proc Natl Acad Sci U S A. 101: 8503-8. PMID: 15159534
2. [Preparation and characterization of monoclonal antibody against Clostridium difficile toxin A].  |  Fu, SW., et al. 2004. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 20: 376-8. PMID: 15193243
3. Simplified purification method for Clostridium difficile toxin A.  |  Fu, SW., et al. 2004. World J Gastroenterol. 10: 2756-8. PMID: 15309736
4. Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB.  |  Gerhard, R., et al. 2005. J Biol Chem. 280: 1499-505. PMID: 15531589
5. Comparison of wild type with recombinant Clostridium difficile toxin A.  |  Gerhard, R., et al. 2005. Microb Pathog. 38: 77-83. PMID: 15748809
6. Clostridium difficile toxin A induces intestinal epithelial cell apoptosis and damage: role of Gln and Ala-Gln in toxin A effects.  |  Brito, GA., et al. 2005. Dig Dis Sci. 50: 1271-8. PMID: 16047471
7. Clostridium difficile toxin A-induced colonocyte apoptosis involves p53-dependent p21(WAF1/CIP1) induction via p38 mitogen-activated protein kinase.  |  Kim, H., et al. 2005. Gastroenterology. 129: 1875-88. PMID: 16344056
8. Crystal structure of receptor-binding C-terminal repeats from Clostridium difficile toxin A.  |  Ho, JG., et al. 2005. Proc Natl Acad Sci U S A. 102: 18373-8. PMID: 16344467
9. Carbohydrate recognition by Clostridium difficile toxin A.  |  Greco, A., et al. 2006. Nat Struct Mol Biol. 13: 460-1. PMID: 16622409

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Clostridium difficile Toxin A Antibody (PCG4) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Clostridium difficile Toxin A Antibody (PCG4) Specifications

Alternate Product Name: Clostridium difficile Toxin A

Product Clone Name: PCG4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: Clostridium difficile Toxin A

Epitope Species: Clostridium difficile

Product Reactivity/Detection: Toxin A

Species Reactivity/Detection: Clostridium difficile

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Clostridium difficile

Applications: ELISA

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Clostridium difficile Toxin A Antibody (PCG4): N/A