CLIM-1/2 Antibody (A-6): sc-376031

CLIM-1/2 Antibody (A-6) is a mouse monoclonal IgG2a antibody that detects CLIM-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIM-1, also known as LIM domain-binding factor-2, plays a crucial role in cellular signaling and transcriptional regulation by interacting with various transcription factors, thereby influencing gene expression. CLIM-1 is primarily located in the cytoplasm, where CLIM-1 is involved in the assembly of higher-order protein complexes that are essential for cellular function and development. The localization of CLIM-1 to actin stress fibers in nonmuscle cells is particularly important, as CLIM-1 facilitates interaction with alpha-actinin-2, a key component of the cytoskeleton, through its PDZ domain. This interaction not only stabilizes the cytoskeletal structure but also plays a significant role in cellular mechanics and signaling pathways. CLIM-1/2 (A-6) antibody serves as an invaluable tool for researchers studying the dynamics of cytoskeletal organization and the regulatory mechanisms of gene expression in various biological contexts.

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CLIM-1/2 Antibody (A-6) References:

1. Islet1 and its co-factor Ldb1 are expressed in quiescent cells of mouse intestinal epithelium.  |  Makarev, E. and Gorivodsky, M. 2014. PLoS One. 9: e95256. PMID: 24755910
2. LDB1-mediated enhancer looping can be established independent of mediator and cohesin.  |  Krivega, I. and Dean, A. 2017. Nucleic Acids Res. 45: 8255-8268. PMID: 28520978
3. The LDB1 Complex Co-opts CTCF for Erythroid Lineage-Specific Long-Range Enhancer Interactions.  |  Lee, J., et al. 2017. Cell Rep. 19: 2490-2502. PMID: 28636938
4. LDB2 inhibits proliferation and migration in liver cancer cells by abrogating HEY1 expression.  |  Yu, H., et al. 2017. Oncotarget. 8: 94440-94449. PMID: 29212240
5. Ldb1 is required for Lmo2 oncogene-induced thymocyte self-renewal and T-cell acute lymphoblastic leukemia.  |  Li, L., et al. 2020. Blood. 135: 2252-2265. PMID: 32181817
6. An evolutionarily conserved Lhx2-Ldb1 interaction regulates the acquisition of hippocampal cell fate and regional identity.  |  Kinare, V., et al. 2020. Development. 147: PMID: 32994168
7. The Ldb1 transcriptional co-regulator is required for establishment and maintenance of the pancreatic endocrine lineage.  |  Toren, E., et al. 2022. FASEB J. 36: e22460. PMID: 35881062
8. Enhancer looping protein LDB1 regulates hepatocyte gene expression by cooperating with liver transcription factors.  |  Liu, G., et al. 2022. Nucleic Acids Res. 50: 9195-9211. PMID: 36018801
9. Serum methylation of GALNT9, UPF3A, WARS, and LDB2 as noninvasive biomarkers for the early detection of colorectal cancer and advanced adenomas.  |  Gallardo-Gómez, M., et al. 2023. Clin Epigenetics. 15: 157. PMID: 37794510
10. LDB1 establishes multi-enhancer networks to regulate gene expression.  |  Aboreden, NG., et al. 2025. Mol Cell. 85: 376-393.e9. PMID: 39721581

The preferred CLIM-2 antibody is CLIM-2 Antibody (C-9): sc-365074.