cleaved Thrombin APII Antibody (G-1): sc-398035
- cleaved Thrombin APII Antibody (G-1) is a mouse monoclonal IgG2b κ cleaved Thrombin APII antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 317-326 a short amino acid sequence containing the neoepitope at Arg 323 of Thrombin Activation Peptide 2 of rat origin
- cleaved Thrombin APII Antibody (G-1) is recommended for detection of cleaved Thrombin APII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-cleaved Thrombin APII Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cleaved Thrombin APII Antibody (G-1). This work is in progress.
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Cleaved Thrombin APII Antibody (G-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects cleaved Thrombin APII protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Cleaved Thrombin APII (G-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cleaved Thrombin APII protein, also known as Prothrombin fragment amino acid 201–324, plays a crucial role in the coagulation cascade, where generation occurs from proteolytic cleavage of Prothrombin, or Coagulation Factor 2. This serine protease is essential for hemostasis, catalyzing fibrinogen conversion to fibrin for clot formation while regulating various cellular processes such as mitogenesis and platelet aggregation. Cleaved Thrombin APII undergoes post-translational modifications, including glycosylation and phosphorylation, which influence stability and protein interactions. These modifications affect Cleaved Thrombin APII′s role in pathological conditions such as thrombosis and cancer metastasis, making anti-Cleaved Thrombin APII antibody (G-1) an invaluable tool for researchers studying coagulation and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cleaved Thrombin APII Antibody (G-1) References:
- Detection of conformational transformation of antithrombin in blood with crossed immunoelectrophoresis: new application for a classical method. | Corral, J., et al. 2003. J Lab Clin Med. 142: 298-305. PMID: 14647033
- A 14 amino acid peptide derived from the amino terminus of the cleaved thrombin receptor elevates intracellular calcium and stimulates prostacyclin production in human endothelial cells. | Ngaiza, JR. and Jaffe, EA. 1991. Biochem Biophys Res Commun. 179: 1656-61. PMID: 1656962
- Trafficking thrombin receptors Biodiversity on the receptor superhighway. | Brass, LF., et al. 1995. Trends Cardiovasc Med. 5: 123-8. PMID: 21232248
- Thrombin decreases expression of the glutamate transporter GLAST and inhibits glutamate uptake in primary cortical astrocytes via the Rho kinase pathway. | Piao, C., et al. 2015. Exp Neurol. 273: 288-300. PMID: 26391563
- The role of thrombin in haemostasis. | Al-Amer, OM. 2022. Blood Coagul Fibrinolysis. 33: 145-148. PMID: 35239615
- Thrombin receptor-activating peptides differentially stimulate platelet-derived growth factor production, monocytic cell adhesion, and E-selectin expression in human umbilical vein endothelial cells. | Shankar, R., et al. 1994. J Biol Chem. 269: 13936-41. PMID: 7514596
- Kinetics of thrombin receptor cleavage on intact cells. Relation to signaling. | Ishii, K., et al. 1993. J Biol Chem. 268: 9780-6. PMID: 7683662
- Interaction of von Willebrand factor with platelets activated by thrombin or a synthetic 7-amino acid peptide derived from the cleaved thrombin receptor. | Minckes, O., et al. 1994. C R Acad Sci III. 317: 363-70. PMID: 8000916
- Receptor mechanism of thrombin-mediated pulmonary vasodilation in neonates. | Pinheiro, JM., et al. 1993. Am J Physiol. 265: L355-9. PMID: 8238369
- Thrombin stimulates atrial natriuretic peptide secretion from rat cardiac atrium. | Klapper, L., et al. 1996. J Pharmacol Exp Ther. 278: 476-81. PMID: 8768694
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cleaved Thrombin APII Antibody (G-1) | sc-398035 | 200 µg/ml | $316.00 | |||
cleaved Thrombin APII Antibody (G-1) AC | sc-398035 AC | 500 µg/ml, 25% agarose | $416.00 | |||
cleaved Thrombin APII Antibody (G-1) HRP | sc-398035 HRP | 200 µg/ml | $316.00 | |||
cleaved Thrombin APII Antibody (G-1) FITC | sc-398035 FITC | 200 µg/ml | $330.00 | |||
cleaved Thrombin APII Antibody (G-1) PE | sc-398035 PE | 200 µg/ml | $343.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 488 | sc-398035 AF488 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 546 | sc-398035 AF546 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 594 | sc-398035 AF594 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 647 | sc-398035 AF647 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 680 | sc-398035 AF680 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII Antibody (G-1) Alexa Fluor® 790 | sc-398035 AF790 | 200 µg/ml | $357.00 | |||
cleaved Thrombin APII (G-1) Neutralizing Peptide | sc-398035 P | 100 µg/0.5 ml | $68.00 |