Date published: 2025-9-5

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Anticorps PARP1 (194C1439): sc-56196

4.3(26)
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Fiches techniques
  • L'anticorps PARP1 194C1439 est un monoclonal IgG2b L'Anticorps PARP1, cité dans 131 publications, fourni en 50 µg/ 0.5 ml
  • soulevée contre un peptide synthétique dont l'épitope se situe près du site de clivage C-terminal de human. PARP-1
  • recommandé pour la détection de cleavad product of PARP-1 d'origine mouse, rat et human par WB et IP
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de PARP1 (194C1439): sc-56196.
  • 2b BP-HRP">m-IgG2b BP-HRP est le réactif de détection secondaire préféré pour l'anticorps PARP1 (194C1439) pour les applications WB. Ce réactif est désormais proposé en association avec l'anticorps PARP1 (194C1439)(voir les informations relatives à la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps PARP1 (194C1439)

sc-56196
50 µg/ 0.5 ml
$316.00

PARP1 (194C1439): m-IgG2b BP-HRP Kit

sc-548435
50 µg Ab; 10 µg BP
$354.00

该抗体在K562中是否能检测总parp?

Posée par: Evelina
感谢您的提问。您可以拨打电话:021-60936350,发送邮件到asia@scbio.cn,或者我们官网上的在线聊天,联系我们的亚洲技术支持团队,了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Répondue par: Technical Service SH
Date de publication: 2022-12-16

Can you recommend a positive control to use with sc-56196:cleaved PARP-1 (194C1439) for Western blot?

Posée par: jenni
We recommend HeLa: sc-2200, Jurkat: sc-2204 or IMR-32: sc-2409 whole cell lysates as we have great Western blot data showing specific detection of cleaved PARP-1 in these cell lines.
Répondue par: Technical Support 13
Date de publication: 2017-01-04
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Rated 4 de 5 de par Anti-cleaved PARP1 per analisi in western blotL'Ab è stato testato utilizzando su lisati cellulari di linee cellulari di tumore mammario ottenuti dopo lisi RIPA buffer. 30 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A. Il secondario 1:2000 in Blotto A. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso.
Date de publication: 2025-01-15
Rated 1 de 5 de par Extremely weak signalThis antibody gives a signal corresponding to cleaved PARP-1 (observed molecular weight: 89 kDa) but, unfortunately, this signal is extremely weak and sometimes not perceptible, even by using positive controls (staurosporine-treated cells) and high resolution ECL. We tested it at several working dilutions (ranging from 1:1.000 to 1:200) and we always obtained similar results. Notably, cleaved PARP1 was clearly visible in the same experimental conditions (40 ug of protein lysates from murine N1E-115 and human SH-SY5Y) by using another commercial antibody.
Date de publication: 2021-12-12
Rated 1 de 5 de par Awful. inespecificWe bought this antibody two weeks ago, for this experiment, but clearly we have waste the money
Date de publication: 2021-03-30
Rated 4 de 5 de par Good antibody for human melanoma cellsThe antibody diluited 1:200 worked well in human melanoma cells
Date de publication: 2020-02-03
Rated 5 de 5 de par wonderful antibody!1:200 diluted for wb of rat protein,the band is quite strong and special
Date de publication: 2018-05-15
Rated 5 de 5 de par mouse heart tissue extracts.The antibody worked really well in the mouse heart tissue extracts.
Date de publication: 2018-03-08
Rated 4 de 5 de par takes some effort12% gel; K562 whole cell lysate; Western Blot 1:200, 4 degrees C overnight, high resolution ECL. First lane: untreated K562, 27ug; second lane: staurosporine treated K562, 20ug; third lane: staurosporine treated K562, 27ug.
Date de publication: 2018-01-08
Rated 4 de 5 de par Bonne detectionJ'ai testé un échantillon de cet anticorps en WB et je suis satisfait du résultat.
Date de publication: 2017-11-28
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Anticorps PARP1 (194C1439) est évalué 4.3 de 5 de 26.
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