Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CLC-2 Double Nickase Plasmid (h): sc-403513-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLC-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLC-2 Double Nickase Plasmid (h) and CLC-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLCN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLC-2 Antibody (D-6): sc-377284
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLC-2 Double Nickase Plasmid (h) | sc-403513-NIC | 20 µg | $410.00 | |||
CLC-2 Double Nickase Plasmid (h2) | sc-403513-NIC-2 | 20 µg | $410.00 |
CLCN2 encodes the voltage-gated chloride channel CLC-2, a plasma membrane anion channel that contributes to chloride homeostasis, membrane excitability, and regulation of epithelial and neuronal ion transport. CLC-2 activity influences cell volume control, transepithelial fluid movement, and electrical stability through coordination with other transporters and channels in processes such as osmotic balance and barrier function. Altered CLCN2 function has been linked to disorders involving epithelial transport and nervous system physiology, supporting its relevance in studying channelopathies and tissue-specific ion flux regulation. As a broadly expressed channel, CLC-2 is frequently investigated in contexts including epithelial physiology, glial and neuronal signaling, and mechanisms that couple ion gradients to cellular stress responses.
CLC-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLCN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLCN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLCN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLCN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.