CLC-1 CRISPR/Cas9 KO Plasmid (h): sc-405071
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLC-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CLC-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLC-1 CRISPR/Cas9 KO Plasmid (h) | sc-405071 | 20 µg | $397.00 |
Overview
CLCN1 encodes the skeletal muscle voltage-gated chloride channel CLC-1, a key determinant of membrane repolarization and electrical stability by providing high resting chloride conductance. By limiting repetitive action potential firing and shaping excitability, CLC-1 supports normal excitation–contraction coupling and muscle fiber homeostasis. CLCN1 activity is regulated by voltage, intracellular chloride, and signaling inputs that alter channel gating, linking it to ion transport physiology and metabolic state. Pathogenic variants or dysregulation of CLCN1 are strongly associated with myotonia and related neuromuscular phenotypes characterized by impaired muscle relaxation.
CLC-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CLCN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CLCN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CLCN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CLC-1 protein expression.
This CRISPR knockout system enables efficient generation of CLCN1-deficient cell models for investigation of CLC-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CLCN1 exon(s) critical for CLC-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CLCN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CLC-1 CRISPR/Cas9 KO Plasmid (h) and CLC-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CLCN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CLC-1 HDR Plasmid (h) and CLC-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CLCN1 homology arms to support homology-directed repair at defined CLCN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.