citrin Double Nickase Plasmid (m): sc-424510-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- citrin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- citrin Double Nickase Plasmid (m) and citrin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc25a13. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: citrin Antibody (D-7): sc-393303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
citrin Double Nickase Plasmid (m) | sc-424510-NIC | 20 µg | $410.00 | |||
citrin Double Nickase Plasmid (m2) | sc-424510-NIC-2 | 20 µg | $410.00 |
Mouse Slc25a13 encodes citrin, a mitochondrial inner membrane aspartate–glutamate carrier that supports the malate–aspartate shuttle and links cytosolic and mitochondrial redox balance to intermediary metabolism. By exchanging aspartate and glutamate across the inner membrane, citrin helps coordinate amino acid metabolism, urea cycle-linked nitrogen handling, and gluconeogenic flux through aspartate availability. Altered SLC25A13 function is associated with metabolic dysregulation phenotypes in model systems, making Slc25a13 a useful node for studying mitochondrial transport, hepatocyte energy metabolism, and systemic nutrient homeostasis. In mice, perturbation of citrin-dependent transport provides a tractable approach to interrogate pathways coupling mitochondrial carrier activity to redox state and nitrogen metabolism.
citrin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc25a13 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc25a13. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc25a13 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc25a13-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.