citrin Antibody (D-7): sc-393303

Citrin Antibody (D-7) is a mouse monoclonal IgA kappa light chain antibody that detects citrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-citrin antibody (D-7) is available as the non-conjugated form. Citrin, also known as SLC25A13 (Solute carrier family 25 member 13), ARALAR2, or CTLN2, is a 675 amino acid multi-pass membrane protein primarily localized to the inner membrane of the mitochondrion. This localization enables citrin to transport calcium-dependent glutamate and aspartate, playing a significant role in the urea cycle, which is essential for nitrogen metabolism and detoxification in the liver. Citrin protein is expressed in various tissues, including the liver, pancreas, kidney, brain, heart, and placenta, highlighting its importance in multiple physiological processes. Citrin features three Solcar repeats and four EF-hand domains that facilitate calcium binding as a member of the mitochondrial carrier family. Mutations in citrin can lead to serious metabolic disorders such as citrullinemia type 2 (CTLN2) and neonatal intrahepatic cholestasis due to citrin deficiency (NICCD). CTLN2 is an autosomal recessive condition characterized by neuropsychiatric symptoms, including memory loss, seizures, and coma, while NICCD manifests during infancy with symptoms such as low birth weight, reduced bile flow, growth retardation, and hepatic fibrosis.

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citrin Antibody (D-7) References:

1. The gene mutated in adult-onset type II citrullinaemia encodes a putative mitochondrial carrier protein.  |  Kobayashi, K., et al. 1999. Nat Genet. 22: 159-63. PMID: 10369257
2. Genomic structure of the adult-onset type II citrullinemia gene, SLC25A13, and cloning and expression of its mouse homologue.  |  Sinasac, DS., et al. 1999. Genomics. 62: 289-92. PMID: 10610724
3. Characterization of a second member of the subfamily of calcium-binding mitochondrial carriers expressed in human non-excitable tissues.  |  Del Arco, A., et al. 2000. Biochem J. 345 Pt 3: 725-32. PMID: 10642534
4. Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria.  |  Palmieri, L., et al. 2001. EMBO J. 20: 5060-9. PMID: 11566871
5. Screening of SLC25A13 mutations in early and late onset patients with citrin deficiency and in the Japanese population: Identification of two novel mutations and establishment of multiple DNA diagnosis methods for nine mutations.  |  Yamaguchi, N., et al. 2002. Hum Mutat. 19: 122-30. PMID: 11793471
6. Mitochondrial aspartate glutamate carrier (citrin) deficiency as the cause of adult-onset type II citrullinemia (CTLN2) and idiopathic neonatal hepatitis (NICCD).  |  Saheki, T. and Kobayashi, K. 2002. J Hum Genet. 47: 333-41. PMID: 12111366
7. Frequency and distribution in East Asia of 12 mutations identified in the SLC25A13 gene of Japanese patients with citrin deficiency.  |  Lu, YB., et al. 2005. J Hum Genet. 50: 338-346. PMID: 16059747
8. Adult onset type II citrullinemia as a cause of non-alcoholic steatohepatitis.  |  Takagi, H., et al. 2006. J Hepatol. 44: 236-9. PMID: 16278034
9. Downregulation of citrin, a mitochondrial AGC, is associated with apoptosis of hepatocytes.  |  Sawada, S., et al. 2007. Biochem Biophys Res Commun. 364: 937-44. PMID: 18273444

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-citrin Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-citrin Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

citrin Antibody (D-7) Specifications

Alternate Product Name: citrin

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids: 30-51

Epitope Target: citrin

Epitope Species: human

Product Reactivity/Detection: citrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for citrin Antibody (D-7): P: Blocking Peptide