CIS Antibody (F-9): sc-166326

CIS Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CIS in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CIS Antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CIS, or cytokine-inducible SH2-containing protein, is a critical regulator of cytokine signaling, consisting of 267 amino acids and featuring a single 96 amino acid SH2 domain. This domain is essential for CIS function, as SH2 allows interaction with tyrosine-phosphorylated receptors, such as the β chain of the interleukin-3 receptor and the erythropoietin receptor, thereby modulating downstream signaling pathways. CIS association with these receptors highlights a role in the regulation of cellular responses to cytokines, making CIS a vital component in the signaling cascades that influence cell growth, differentiation, and survival. anti-CIS antibody (F-9) enables researchers to gain valuable insights into the mechanisms of cytokine signaling and implications in various biological processes and diseases.

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CIS Antibody (F-9) References:

1. Regulation and function of the cytokine-inducible SH-2 domain proteins, CIS and SOCS3, in mammary epithelial cells.  |  Tonko-Geymayer, S., et al. 2002. Mol Endocrinol. 16: 1680-95. PMID: 12089360
2. Negative regulation of cytokine signaling by CIS/SOCS family proteins and their roles in inflammatory diseases.  |  Hanada, T., et al. 2003. Rev Physiol Biochem Pharmacol. 149: 72-86. PMID: 12687406
3. Thrombin induces expression of cytokine-induced SH2 protein (CIS) in rat brain astrocytes: involvement of phospholipase A2, cyclooxygenase, and lipoxygenase.  |  Ji, KA., et al. 2004. Glia. 48: 102-11. PMID: 15378659
4. Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalization.  |  Landsman, T. and Waxman, DJ. 2005. J Biol Chem. 280: 37471-80. PMID: 16154995
5. Mechanisms underlying the diminished sensitivity to prolactin negative feedback during lactation: reduced STAT5 signaling and up-regulation of cytokine-inducible SH2 domain-containing protein (CIS) expression in tuberoinfundibular dopaminergic neurons.  |  Anderson, ST., et al. 2006. Endocrinology. 147: 1195-202. PMID: 16357045
6. Increased growth hormone receptor (GHR) degradation due to over-expression of cytokine inducible SH2 domain-containing protein (CIS) as a cause of GH transduction defect (GHTD).  |  Gil, AP., et al. 2012. J Pediatr Endocrinol Metab. 25: 897-908. PMID: 23426819
7. CIS is a potent checkpoint in NK cell-mediated tumor immunity.  |  Delconte, RB., et al. 2016. Nat Immunol. 17: 816-24. PMID: 27213690
8. CIS and TGF-β regulatory pathways influence immunity to bacterial infection.  |  McCulloch, TR., et al. 2022. Immunology. 167: 54-63. PMID: 35611558
9. CIS deletion by CRISPR/Cas9 enhances human primary natural killer cell functions against allogeneic glioblastoma.  |  Nakazawa, T., et al. 2023. J Exp Clin Cancer Res. 42: 205. PMID: 37563692
10. ADSC secretome constrains NK cell activity by attenuating IL-2-mediated JAK-STAT and AKT signaling pathway via upregulation of CIS and DUSP4.  |  Ko, E., et al. 2023. Stem Cell Res Ther. 14: 329. PMID: 37964351

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIS Antibody (F-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CIS Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIS Antibody (F-9) Specifications

Alternate Product Name: CIS

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 10-38

Epitope Target: CIS

Epitope Species: mouse

Product Reactivity/Detection: CIS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CIS Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred CIS antibody is CIS Antibody (D-8): sc-74581.