cis-ACCP is a reversible and competitive inhibitor of type IV collagen-specific MMP-2 and MMP-9 with preference towards MMP-2 (IC50 = 4 and 20 μM, respectively). At 100 μM, cis-ACCP prevents 90% of tumor cell invasion across matrigel-coated membranes in vitro. Matrix metalloproteinases (MMPs) belong to a family of proteases that play a crucial role in tissue repair and remodeling by degrading extracellular matrix proteins, thus enabling cell migration. Overexpression of MMPs are linked to diseases such as arthritis, cancer, osteoporosis and arteriosclerosis.
1 Nagase, H., Woessner, J.F. Jr. Matrix metalloproteinases. J Biol Chem 274(31) 21491-21494 (1999). 2 Hoffman, A., Qadri, B., Frant, J., et al. Carbamoylphosphonate matrix metalloproteinase inhibitors 6: Cis-2-aminocyclohexylcarbamoylphosphonic acid, a novel orally active antimetastatic matrix metalloproteinase-2 selective inhibitor-synthesis and pharmacodynamic and pharmacokinetic analysis. J Med Chem 51 1406-1414 (2008).
cis-ACCP is supplied as a crystalline solid. A stock solution may be made by dissolving the cis-ACCP in an organic solvent purged with an inert gas. cis-ACCP is soluble in ethanol to a concentration of approximately 0.15 mg/ml.
Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of cis-ACCP can be prepared by directly dissolving the crystalline compound in aqueous buffers. The solubility of cis-ACCP in PBS, pH 7.2, is approximately 0.3 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Soluble in ethanol (~0.15 mg/ml), and PBS pH7.2 (~0.3 mg/ml).
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn