CIDE-B Antibody (5X): sc-101244

CIDE-B Antibody (5X) is a mouse monoclonal IgG1 kappa light chain antibody that detects CIDE-B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CIDE-B antibody (5X) is available as the non-conjugated monoclonal isotype antibody. CIDE-B plays a crucial role in the regulation of apoptosis, particularly in the DNA fragmentation process during programmed cell death. CIDE-B interacts with the DNA fragmentation factor (DFF), which consists of two subunits: DFF-45 (also known as ICAD) and CPAN (caspase-activated nuclease). CPAN is a deoxyribonuclease that is essential for the degradation of DNA during apoptosis, and DFF-45 tightly regulates CPAN activity. When caspase-3 is activated, caspase-3 cleaves DFF-45, releasing CPAN to enter the nucleus and initiate DNA degradation. CIDE-B, along with its homolog CIDE-A, shares structural similarities with the N-terminal region of DFF-45 and contributes to the apoptotic process by promoting cell death and DNA fragmentation, while DFF-45 also inhibits CIDE-B. This intricate interplay highlights CIDE-B′s importance in maintaining cellular homeostasis and regulating apoptosis, making CIDE-B a significant target for research in cancer and other diseases where apoptosis is dysregulated.

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CIDE-B Antibody (5X) References:

1. The hepatitis C virus NS2 protein is an inhibitor of CIDE-B-induced apoptosis.  |  Erdtmann, L., et al. 2003. J Biol Chem. 278: 18256-64. PMID: 12595532
2. Cocaine-induced changes in the expression of apoptosis-related genes in the fetal mouse cerebral wall.  |  Novikova, SI., et al. 2005. Neurotoxicol Teratol. 27: 3-14. PMID: 15681117
3. Analysis of differentially expressed genes in nitric oxide-exposed human monocytic cells.  |  Turpaev, K., et al. 2005. Free Radic Biol Med. 38: 1392-400. PMID: 15855057
4. Gene expression profiling of jejunal Peyer's patches in juvenile and adult pigs.  |  Machado, JG., et al. 2005. Mamm Genome. 16: 599-612. PMID: 16180142
5. Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene.  |  Da, L., et al. 2006. Biochem J. 393: 779-88. PMID: 16248853
6. Cell death-inducing DFF45-like effector may take part in neuronal apoptosis of the lumbar spinal cord after sciatic nerve injury caused by a firearm.  |  Wang, JM., et al. 2006. Mil Med. 171: 793-9. PMID: 16933827
7. Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) stimulates VLDL assembly through activation of cell death-inducing DFFA-like effector B (CideB).  |  Chen, Z., et al. 2010. J Biol Chem. 285: 25996-6004. PMID: 20551328
8. Cell death-inducing DFFA-like effector b is required for hepatitis C virus entry into hepatocytes.  |  Wu, X., et al. 2014. J Virol. 88: 8433-44. PMID: 24829338
9. Hepatitis C Virus-Induced Degradation of Cell Death-Inducing DFFA-Like Effector B Leads to Hepatic Lipid Dysregulation.  |  Lee, EM., et al. 2016. J Virol. 90: 4174-85. PMID: 26865724
10. Cell-death-inducing DFFA-like Effector B Contributes to the Assembly of Hepatitis C Virus (HCV) Particles and Interacts with HCV NS5A.  |  Cai, H., et al. 2016. Sci Rep. 6: 27778. PMID: 27282740
11. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor.  |  Inohara, N., et al. 1998. EMBO J. 17: 2526-33. PMID: 9564035
12. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD.  |  Inohara, N., et al. 1999. J Biol Chem. 274: 270-4. PMID: 9867840

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIDE-B Antibody (5X) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CIDE-B Antibody (5X) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIDE-B Antibody (5X) Specifications

Alternate Product Name: CIDE-B

Product Clone Name: 5X

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-111

Epitope Target: CIDE-B

Epitope Species: human

Product Reactivity/Detection: CIDE-B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CIDE-B Antibody (5X): N/A