CIDE-A Antibody (4B9): sc-293289

CIDE-A Antibody (4B9) is a mouse monoclonal IgG2a kappa light chain antibody that detects CIDE-A protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CIDE-A antibody (4B9) is available as the non-conjugated monoclonal isotype antibody. CIDE-A protein plays a crucial role in the regulation of apoptosis, specifically by promoting cell death and DNA fragmentation, which is essential for maintaining cellular homeostasis and eliminating damaged cells. CIDE-A protein interacts with the DNA fragmentation factor (DFF), which consists of two subunits: DFF-45, an inhibitor of caspase-activated DNase (CAD), and CPAN, the active DNase responsible for DNA degradation during apoptosis. The interaction between CIDE-A protein and DFF-45 is significant because this highlights the regulatory mechanisms that control apoptosis; CIDE-A protein, along with CIDE-B homolog, can enhance the apoptotic process by promoting the release of CPAN from DFF-45, thereby facilitating DNA degradation. Understanding CIDE-A protein function and interactions is vital for elucidating the pathways involved in programmed cell death, which has implications in cancer research and therapeutic strategies.

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CIDE-A Antibody (4B9) References:

1. CIDE-A, a novel link between brown adipose tissue and obesity.  |  Lin, SC. and Li, P. 2004. Trends Mol Med. 10: 434-9. PMID: 15350895
2. Cocaine-induced changes in the expression of apoptosis-related genes in the fetal mouse cerebral wall.  |  Novikova, SI., et al. 2005. Neurotoxicol Teratol. 27: 3-14. PMID: 15681117
3. Cide-a and Cide-c are induced in the progression of hepatic steatosis and inhibited by eicosapentaenoic acid.  |  Jinno, Y., et al. 2010. Prostaglandins Leukot Essent Fatty Acids. 83: 75-81. PMID: 20542418
4. Structural insight into CIDE domains: the Janus face of CIDEs.  |  Park, HH. 2015. Apoptosis. 20: 240-9. PMID: 25488366
5. Cell death-associated lipid droplet protein CIDE-A is a noncanonical marker of endoplasmic reticulum stress.  |  Morishita, Y., et al. 2021. JCI Insight. 6: PMID: 33661766
6. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.  |  Liu, X., et al. 1997. Cell. 89: 175-84. PMID: 9108473
7. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.  |  Enari, M., et al. 1998. Nature. 391: 43-50. PMID: 9422506
8. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.  |  Sakahira, H., et al. 1998. Nature. 391: 96-9. PMID: 9422513
9. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45.  |  Halenbeck, R., et al. 1998. Curr Biol. 8: 537-40. PMID: 9560346
10. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor.  |  Inohara, N., et al. 1998. EMBO J. 17: 2526-33. PMID: 9564035
11. Identification of the nuclear factor HMG2 as an activator for DFF nuclease activity.  |  Toh, SY., et al. 1998. Biochem Biophys Res Commun. 250: 598-601. PMID: 9784391
12. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD.  |  Inohara, N., et al. 1999. J Biol Chem. 274: 270-4. PMID: 9867840

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CIDE-A Antibody (4B9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CIDE-A Antibody (4B9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CIDE-A Antibody (4B9) Specifications

Alternate Product Name: CIDE-A

Product Clone Name: 4B9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-253

Epitope Target: CIDE-A

Epitope Species: human

Product Reactivity/Detection: CIDE-A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CIDE-A Antibody (4B9): N/A