Chromeo P503 An amine-reactive pyrylium dye.

Chromeo™ P503

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 126ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Application: Chromeo™ P503 is an amine-reactive pyrylium dye
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Chromeo™ P503 detects proteins and peptides by exhibiting a color change from blue to red. After binding to primary amines, it undergos a large shortwave spectral shift of more than 100nm that is visible to the naked eye. There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. The shift of the absorption & emission bands together with the increased fluorescence quantum yield effectively eliminates background from any unbound dye. In addition, because unbound the pyrylium dye is hydrolyzed during the labeling procedure, there is no need for purification following the simple one-step, room-temperature incubation. The labeled protein is ready to use immediately, and any possible background from the unbound dye is eliminated. Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point. Because of their unique features, Chromeo Py-Dyes have been used successfully in a number of "no-wash" applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies.


References

1. Craig, D.B. et al. 2005. Electrophoresis. 26(11): 2208-2213. PMID: 15880625
2. Hoffmann, K. et al. 2008. Ann. N. Y. Acad. Sci. 1130 : 28-34. PMID: 18596328
3. Swearingen, K.E. et al. 2008. J Chromatogr A. 1194(2): 249-252. PMID: 18479693
4. Turner, E.H. et al. 2008. J Chromatogr A. 1194(2): 253-256. PMID: 18482729
5. Ramsay, L.M. et al. 2009. Anal. Chem. 81(5): 1741-1746. PMID: 19206532
6. Ramsay, L.M. et al. 2009. Electrophoresis. 30(2): 297-302. PMID: 19204946
7. Dada, O.O. et al. 2010. Anal Bioanal Chem. 397(8): 3305-3310. PMID: 20336452
8. Dickerson, J.A. et al. 2010. Electrophoresis. 31(15): 2650-2654. PMID: 20603830
9. de Jong, S. and Krylov, S.N. 2011. Anal. Chem. 83(16): 6330-6335. PMID: 21728308
10. Keller, M. et al. 2011. Bioorg. Med. Chem. 19(9): 2859-2878. PMID: 21493077
11. de Jong, S. and Krylov, S.N. 2012. Anal. Chem. 84(1): 453-458. PMID: 22107082
12. Johannesen, S.A. et al. 2012. Carbohydr. Res. 352 : 94-100. PMID: 22425443

Appearance :
Powder
Physical State :
Solid
Storage :
Store at 4° C
Fluoresence :
λex 612 nm, λem 655 nm; λex 503 nm, λem 600 nm (conjugated)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 125ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.