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Chlorophenol Red-β-D-galactopyranoside (CAS 99792-79-7)

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Alternate Names:
CPRG, Chlorophenol Red-β-D-galactoside
Application:
Chlorophenol Red-β-D-galactopyranoside is a chemical which measures the B-galactosidase expression levels in transfected cells
CAS Number:
99792-79-7
Purity:
≥90%
Molecular Weight:
585.41
Molecular Formula:
C25H22Cl2O10S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Chlorophenol Red-β-D-galactopyranoside measures beta-galactosidase expression levels in transfected cells. LacZ gene is a popular reporter gene in transfection reactions because its product, beta-galactosidase, is very stable. Chlorophenol Red-β-D-galactopyranoside is a substrate of beta-Galactosidase.


Chlorophenol Red-β-D-galactopyranoside (CAS 99792-79-7) References

  1. Measurement of beta-galactosidase tissue levels in a tumor cell xenograft model.  |  Buller, CJ., et al. 2003. Methods Find Exp Clin Pharmacol. 25: 713-6. PMID: 14685298
  2. Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors.  |  Yang, X., et al. 2005. Anal Biochem. 336: 102-7. PMID: 15582564
  3. Freeze-dried recombinant bacteria for on-site detection of phenolic compounds by color change.  |  Shin, HJ., et al. 2005. J Biotechnol. 119: 36-43. PMID: 16051389
  4. The effect of physico-chemical parameters and chemical compounds on the activity of beta-d-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water.  |  Wutor, VC., et al. 2007. Chemosphere. 68: 622-7. PMID: 17433413
  5. Oral gene delivery with cyclo-(D-Trp-Tyr) peptide nanotubes.  |  Hsieh, WH., et al. 2012. Mol Pharm. 9: 1231-49. PMID: 22480317
  6. Oestrogenic activity of CPRG (chlorophenol red-β-D-galactopyranoside), a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays.  |  Els Vanderperren Wim Demaré Ronny Blust Kris Cooreman Peter Bossier,. 2001. Biomarkers. 6: 375-80. PMID: 23889310
  7. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-D-galactopyranoside.  |  Sicard, C., et al. 2014. Anal Bioanal Chem. 406: 5395-403. PMID: 24972873
  8. Detection of Escherichia coli in drinking water using T7 bacteriophage-conjugated magnetic probe.  |  Chen, J., et al. 2015. Anal Chem. 87: 8977-84. PMID: 26172120
  9. An improved CPRG colorimetric ligand-receptor signal transduction assay based on beta-galactosidase activity in mammalian BWZ-reporter cells.  |  Jendresen, C., et al. 2018. J Pharmacol Toxicol Methods. 90: 67-75. PMID: 29203451
  10. Rapid Bacteria Detection at Low Concentrations Using Sequential Immunomagnetic Separation and Paper-Based Isotachophoresis.  |  Schaumburg, F., et al. 2019. Anal Chem. 91: 9623-9630. PMID: 31282665
  11. Orthogonal paper biosensor for mercury(II) combining bioluminescence and colorimetric smartphone detection.  |  Lopreside, A., et al. 2021. Biosens Bioelectron. 194: 113569. PMID: 34438340
  12. Development of semi-quantitative urinary sodium test strip.  |  Lai, JS., et al. 2022. Biotechnol Appl Biochem.. PMID: 35830743
  13. Identification of beta-galactosidase activity in purified bovine retinal rod outer segments.  |  Prasad, VV. and Fliesler, SJ. 1994. Curr Eye Res. 13: 377-84. PMID: 8055701

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Chlorophenol Red-β-D-galactopyranoside, 25 mg

sc-257242
25 mg
$59.00

Chlorophenol Red-β-D-galactopyranoside, 100 mg

sc-257242A
100 mg
$184.00

Chlorophenol Red-β-D-galactopyranoside, 500 mg

sc-257242B
500 mg
$668.00

Chlorophenol Red-β-D-galactopyranoside, 1 g

sc-257242C
1 g
$1244.00

Por favor, me podrían indicar en qué se disuelve el CPRG?

Asked by: Julio
Gracias por tu pregunta. sc-257242: Chlorophenol Red-β-D-galactopyranoside es soluble en agua hasta 20 mg/mL. Thank you for your question. sc-257242:Chlorophenol Red-β-D-galactopyranoside is soluble in water up to 20 mg/mL.
Answered by: Technical Support
Date published: 2018-12-31
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Rated 5 out of 5 by from worked greatWorked great for b3z assay for quantifying beta gal from b3z cells
Date published: 2022-01-28
Rated 5 out of 5 by from ExcellentThe reagent arrived in good condition on time and same as usual it is performing very well, homogenously.
Date published: 2020-10-05
Rated 5 out of 5 by from Goodworks great for B3Z cells for antigen processing events
Date published: 2018-05-02
Rated 5 out of 5 by from Various publications cite the use of ChlorophenolVarious publications cite the use of Chlorophenol Red- -D-galactopyranoside to measure the -galactosidase expression levels in transfected cells. -SCBT Publication Review
Date published: 2015-01-16
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Chlorophenol Red-β-D-galactopyranoside is rated 5.0 out of 5 by 4.
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