Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Chk2 Double Nickase Plasmid (h): sc-400438-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Chk2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Chk2 Double Nickase Plasmid (h) and Chk2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHEK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Chk2 Antibody (A-11): sc-17747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Chk2 Double Nickase Plasmid (h) | sc-400438-NIC | 20 µg | $410.00 | |||
Chk2 Double Nickase Plasmid (h2) | sc-400438-NIC-2 | 20 µg | $410.00 |
CHEK2 encodes checkpoint kinase 2 (Chk2), a serine/threonine kinase activated downstream of ATM in response to DNA double-strand breaks. Upon phosphorylation, Chk2 propagates DNA damage signaling to regulate cell-cycle checkpoints, DNA repair coordination, and apoptosis through substrates such as p53, CDC25 phosphatases, and BRCA1. This pathway integrates genomic stress cues with replication control to preserve genome integrity during S phase and mitosis. Dysregulated CHEK2/Chk2 signaling and inherited or acquired variants have been associated with genome instability phenotypes and cancer susceptibility, making it a widely used node for studying DNA damage response circuitry.
Chk2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHEK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHEK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHEK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHEK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.