CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
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See how others have used CHAPS (CAS 75621-03-3). Click on the entry to view the PubMed entry .
PMID: # 29395787 Blaza, JN.|Vinothkumar, KR.|Hirst, J.| et al. 2018. Structure. 26: 312-319.e3.
PMID: # 27940020 Bridges, HR.|Mohammed, K.|Harbour, ME.|Hirst, J.| et al. 2017. Biochim Biophys Acta Bioenerg. 1858: 197-207.
PMID: # 24947230 Tan, ML.|Shao, P.|Friedhuber, AM.|van Moorst, M.|Elahy, M.|Indumathy, S.|Dunstan, DE.|Wei, Y.|Dass, CR.| et al. 2014. Biomaterials. 35: 7828-38.
PMID: # 32478334 Cell Rep Med. 1: 100014.
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