CGI-69 Double Nickase Plasmid (m): sc-426907-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CGI-69 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CGI-69 Double Nickase Plasmid (m) and CGI-69 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc25a39. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CGI-69 Double Nickase Plasmid (m) | sc-426907-NIC | 20 µg | $410.00 |
Slc25a39 encodes the mitochondrial inner membrane carrier protein CGI-69 in mouse, implicated in maintaining mitochondrial redox balance and supporting oxidative metabolism. CGI-69 has been linked to transport processes that influence glutathione homeostasis, iron utilization, and the integrity of respiratory chain function, thereby connecting it to pathways controlling reactive oxygen species and mitochondrial biogenesis. Perturbation of Slc25a39 activity can impact cellular stress responses and energy-demanding tissues, making it relevant to studies of anemia-related phenotypes, neurobiology, and metabolic dysfunction where mitochondrial homeostasis is disrupted. As a conserved mitochondrial carrier, CGI-69 is also useful for dissecting organelle communication and metabolic rewiring during differentiation or stress.
CGI-69 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc25a39 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc25a39. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc25a39 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc25a39-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.