CFSE CAS: 150347-59-4
MF: C29H19NO11
MW: 557.46
A cell permeable amine-reactive fluorescent label.

CFSE (CAS 150347-59-4)

CFSE | CAS 150347-59-4 is rated 5.0 out of 5 by 1.
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Alternate Names: 5(6)-CFDA; 5(6)-Carboxyfluorescein diacetate, NHS ester
Application: CFSE is a cell permeable amine-reactive fluorescent label
CAS Number: 150347-59-4
Molecular Weight: 557.46
Molecular Formula: C29H19NO11
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

CFSE is a cell-permeable amine-reactive fluorescent label for tracking cells for long periods. It has been used to track cell cycle division and immunophenotypic differentiation of haematopoietic stem and progenitor cells. It is non-fluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent fluorophore. The compound has also been used in T-cell proliferation assays. CFSE has been observed to fluoresce at a EX(max) of 495nm and emit at Em(max) of 519 nm allowing to also be used for flow cytometric evaluations.


References

1. Weston, S.A. and Parish, C.R. 1990. J. Immunol. Methods. 133: 87-97. PMID: 2212694
2. De Clerck, L.S., et al. 1994. J. Immunol. Methods. 172: 115-124. PMID: 8207260
3. Oehen, S., et al. 1997. J. Immunol. Methods. 207: 33-42. PMID: 9328584
4. Lyons, A.B., 1999. Immunol. Cell Biol. 77: 509-515. PMID: 10571671
5. Mintern, J., et al. 1999. Immunol. Cell Biol. 77: 539-543. PMID: 10571675
6. Gruber, H.E., et al. 2000. Biotech Histochem. 75: 118-123. PMID: 10950173
7. Lyons, A.B. 2000. J. Immunol. Methods. 243: 147-154. PMID: 10986412
8. Suchin, E.J., et al. 2001. J. Immunol. 166: 973-981. PMID: 11145675

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO, DMF, methanol, and water (Partly Miscible).
Storage :
Store at -20° C
Melting Point :
152-154° C (lit.)
Fluoresence :
λex 492 nm, λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
16211581
MDL Number :
MFCD00037466
SMILES :
CC(=O)OC1=CC=C2C(OC3=CC(OC(C)=O)=CC=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)ON2C(=O)CCC2=O)=C1

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Certificate of Analysis

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CFSE (CAS 150347-59-4) Product Citations

See how others have used CFSE (CAS 150347-59-4). Click on the entry to view the PubMed entry .

Citations 1 to 7 of 7 total

PMID: # 30986544  Li, Q.|Chen, Y.|Zhao, D.|Yang, S.|Zhang, S.|Wei, Z.|Wang, Y.|Qian, K.|Zhao, B.|Zhu, Y.|Chen, Y.|Duan, Y.|Han, J.|Yang, X.| et al. 2019. Pharmacol. Res. 144: 167-180.

PMID: # 27184189  Saini, A. et al. 2016. Eur. J. Immunol. 46: 1842-53.

PMID: # 26712205  Weidensdorfer, M. et al. 2015. Infect. Immun. 84: 711-22.

PMID: # 24292068  Hudak, JE. et al. 2014. Nat. Chem. Biol. 10: 69-75.

PMID: # 15240681  Chemnitz, JM. et al. 2004. J. Immunol. 173: 945-954.

PMID: # 15308576  Rivollier A et al. 2004. Blood. 104(13): 4029-4037.

PMID: # 22483852  Shotorbani, SS. et al. Cell. Immunol. 275: 61-8.

Citations 1 to 7 of 7 total

Is CFSE (CAS 150347-59-4) sensitive to formaldehyde fixation?

Asked by: mr783
Thank you for your question. It is not recommended to use any type of fixation in conjunction with CFSE. CFSE is a cell-permeable amine-reactive fluorescent label for tracking cells for long periods. Please refer to the product description, and references, on our website, below the ordering information for more detailed information.
Answered by: Technical Support
Date published: 2019-01-18

How should this be stored after dissolving in DMSO?

Asked by: SCM4
This chemical, CFSE (CAS 150347-59-4), can be stored long term, we recommend storing the product in its original state, as a solid, at -20ºC. For short term storage of a solution, aliquot and store at -20ºC. In either case, avoid repeated freeze/thaw cycles.
Answered by: Chemical Support 1
Date published: 2019-01-18
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Rated 5 out of 5 by from Chemnitz Chemnitz, et. al. (PubMed ID 15240681) used CFSE to measure the accumulation of cells within the culture. -SCBT Publication Review
Date published: 2015-03-01
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