Date published: 2026-4-24

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Cephalexin-d5 (CAS 23325-78-2 (unlabeled))

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Alternate Names:
(6R,7R)-7-[[(2R)-2-Amino-2-(phenyl-d5)acetyl]amino]-3-methyl-8-oxo -5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid
CAS Number:
23325-78-2 (unlabeled)
Molecular Weight:
370.43
Molecular Formula:
C16H14D5N3O5S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Cephalexin-d5 is a deuterated analog of cephalexin where five hydrogen atoms are replaced with deuterium. This subtle yet impactful modification significantly enhances the utility of cephalexin in non-clinical research applications, particularly within the realms of analytical chemistry and environmental studies. The incorporation of deuterium, a stable isotope of hydrogen, alters the mass of the molecule without changing its chemical structure, enabling more precise detection and quantification in mass spectrometry analyses. This property is especially vital when differentiating the deuterated compound from its non-deuterated counterpart in complex biochemical or environmental matrices. Cephalexin-d5 is extensively used in method development for the accurate monitoring of cephalexin in environmental samples, such as water bodies and soils, providing critical insights into the antibiotic′s behavior, such as its degradation pathways and persistence in natural settings. The enhanced detection capabilities allow researchers to trace minute quantities of cephalexin, thereby contributing to studies on its environmental impact and the potential for contributing to antibiotic resistance in microbial communities. Furthermore, cephalexin-d5 serves as a precise internal standard in analytical procedures, ensuring high reproducibility and reliability in experimental outcomes, which is crucial for advancing the understanding of how such substances interact with and affect ecosystems.


Cephalexin-d5 (CAS 23325-78-2 (unlabeled)) References

  1. Impact of Chemical Structure on Conjunctival Drug Permeability: Adopting Porcine Conjunctiva and Cassette Dosing for Construction of In Silico Model.  |  Ramsay, E., et al. 2017. J Pharm Sci. 106: 2463-2471. PMID: 28479360
  2. Melanin binding study of clinical drugs with cassette dosing and rapid equilibrium dialysis inserts.  |  Pelkonen, L., et al. 2017. Eur J Pharm Sci. 109: 162-168. PMID: 28756205
  3. Binding of Small Molecule Drugs to Porcine Vitreous Humor.  |  Rimpelä, AK., et al. 2018. Mol Pharm. 15: 2174-2179. PMID: 29648838
  4. Determination of cephalexin residual level using ultra-high-performance liquid chromatography-tandem mass spectrometry: Residue depletion study in swine.  |  Wang, L., et al. 2019. J Chromatogr B Analyt Technol Biomed Life Sci. 1124: 233-238. PMID: 31233944
  5. Antibiotics in coastal water and sediments of the East China Sea: Distribution, ecological risk assessment and indicators screening.  |  Li, F., et al. 2020. Mar Pollut Bull. 151: 110810. PMID: 32056603
  6. Occurrence of Antibiotics in Influent and Effluent from 3 Major Wastewater-Treatment Plants in Finland.  |  Kortesmäki, E., et al. 2020. Environ Toxicol Chem. 39: 1774-1789. PMID: 32557762
  7. Fate of antibiotics in engineered wastewater systems and receiving water environment: A case study on the coast of Hangzhou Bay, China.  |  Bao, Y., et al. 2021. Sci Total Environ. 769: 144642. PMID: 33736269
  8. Assessment of Cold Atmospheric Pressure Plasma (CAPP) Treatment for Degradation of Antibiotic Residues in Water.  |  Wielogorska, E., et al. 2023. Antibiotics (Basel). 12: PMID: 37508211

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cephalexin-d5, 1 mg

sc-217872
1 mg
$448.00