CEP350 Double Nickase Plasmid (h): sc-406478-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEP350 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CEP350 Double Nickase Plasmid (h) and CEP350 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CEP350. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEP350 Double Nickase Plasmid (h) | sc-406478-NIC | 20 µg | $410.00 |
CEP350 (centrosomal protein 350) is a large coiled-coil centrosome-associated scaffold that localizes predominantly to the centrosome and mother centriole, where it helps organize microtubule nucleation and anchoring. Through interactions with centrosomal and Golgi-associated factors, CEP350 contributes to centrosome integrity, spindle organization, and cilia-related processes that depend on accurate centriole function. Proper CEP350 activity supports cell-cycle progression and genome stability by coordinating centrosome duplication and mitotic microtubule dynamics. Dysregulation of centrosome architecture and microtubule organization involving CEP350 is relevant to studies of chromosomal instability and ciliopathy-associated cellular phenotypes.
CEP350 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CEP350 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CEP350. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CEP350 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CEP350-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.