CENP-C Antibody (C-6): sc-166099
- CENP-C Antibody (C-6) is a mouse monoclonal IgG2b κ CENP-C antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 644-943 mapping at the C-terminus of CENP-C of human origin
- CENP-C Antibody (C-6) is recommended for detection of CENP-C of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CENP-C Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for CENP-C Antibody (C-6) for WB applications. This reagent is now offered in a bundle with CENP-C Antibody (C-6) (see ordering information below).
CENP-C Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects CENP-C protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CENP-C antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CENP-C plays a crucial role in the assembly and function of kinetochores, which are essential for accurate chromosome segregation during cell division. Located on the fibers of the kinetochore, CENP-C serves as a vital organizing center that tightly associates with DNA, ensuring proper formation of a functional centromere. Any disruption in CENP-C function can lead to errors in mitotic progression, potentially resulting in aneuploidy and various diseases, including cancer. Studies have shown that CENP-C is lost from centromeres during herpes simplex virus 1 infection, leading to significant structural changes in the kinetochore, indicating that CENP-C regulation is critical throughout the cell cycle.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CENP-C Antibody (C-6) References:
- Specific destruction of kinetochore protein CENP-C and disruption of cell division by herpes simplex virus immediate-early protein Vmw110. | Everett, RD., et al. 1999. EMBO J. 18: 1526-38. PMID: 10075924
- Visualization of prekinetochore locus on the centromeric region of highly extended chromatin fibers: does kinetochore autoantigen CENP-C constitute a kinetochore organizing center? | Sugimoto, K., et al. 1999. Chromosome Res. 7: 9-19. PMID: 10219728
- A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore. | Dawe, RK., et al. 1999. Plant Cell. 11: 1227-38. PMID: 10402425
- CENP-C is necessary but not sufficient to induce formation of a functional centromere. | Fukagawa, T., et al. 1999. EMBO J. 18: 4196-209. PMID: 10428958
- Centromerization. | Choo, KH. 2000. Trends Cell Biol. 10: 182-8. PMID: 10754560
- CENP-C unwraps the human CENP-A nucleosome through the H2A C-terminal tail. | Ali-Ahmad, A., et al. 2019. EMBO Rep. 20: e48913. PMID: 31475439
- CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization. | Watanabe, R., et al. 2019. J Cell Biol. 218: 4042-4062. PMID: 31676716
- Cryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C. | Ariyoshi, M., et al. 2021. EMBO J. 40: e105671. PMID: 33463726
- Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice. | Fan, J., et al. 2021. J Immunol Res. 2021: 4610494. PMID: 33604391
- Centromere/kinetochore is assembled through CENP-C oligomerization. | Hara, M., et al. 2023. Mol Cell. 83: 2188-2205.e13. PMID: 37295434
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation. | Fellmeth, JE., et al. 2023. PLoS Genet. 19: e1011066. PMID: 38019881
- The vertebrate cell kinetochore and its roles during mitosis. | Rieder, CL. and Salmon, ED. 1998. Trends Cell Biol. 8: 310-8. PMID: 9704407
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-C Antibody (C-6) | sc-166099 | 200 µg/ml | $322.00 | |||
CENP-C Antibody (C-6): m-IgG2b BP-HRP Bundle | sc-548673 | 200 µg Ab; 10 µg BP | $361.00 | |||
CENP-C Antibody (C-6) AC | sc-166099 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CENP-C Antibody (C-6) HRP | sc-166099 HRP | 200 µg/ml | $322.00 | |||
CENP-C Antibody (C-6) FITC | sc-166099 FITC | 200 µg/ml | $336.00 | |||
CENP-C Antibody (C-6) PE | sc-166099 PE | 200 µg/ml | $349.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 488 | sc-166099 AF488 | 200 µg/ml | $364.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 546 | sc-166099 AF546 | 200 µg/ml | $364.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 594 | sc-166099 AF594 | 200 µg/ml | $364.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 647 | sc-166099 AF647 | 200 µg/ml | $364.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 680 | sc-166099 AF680 | 200 µg/ml | $364.00 | |||
CENP-C Antibody (C-6) Alexa Fluor® 790 | sc-166099 AF790 | 200 µg/ml | $364.00 |