Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CEACAM5 Double Nickase Plasmid (h): sc-401219-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEACAM5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CEACAM5 Double Nickase Plasmid (h) and CEACAM5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CEACAM5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CEACAM5 Antibody (CI-P83-1): sc-23928
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEACAM5 Double Nickase Plasmid (h) | sc-401219-NIC | 20 µg | $410.00 | |||
CEACAM5 Double Nickase Plasmid (h2) | sc-401219-NIC-2 | 20 µg | $410.00 |
CEACAM5 (carcinoembryonic antigen-related cell adhesion molecule 5, CD66e) is a GPI-anchored immunoglobulin superfamily glycoprotein that mediates cell–cell adhesion and participates in epithelial organization and tissue architecture. It influences signaling events linked to cell polarity, differentiation, and contact-dependent growth control, and can modulate interactions between tumor cells and the microenvironment. CEACAM5 is widely studied as an epithelial lineage marker and is frequently dysregulated in gastrointestinal and other carcinomas, where altered expression correlates with tumor progression phenotypes. Its biology intersects with pathways governing adhesion dynamics, immune recognition at mucosal surfaces, and metastatic dissemination processes.
CEACAM5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CEACAM5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CEACAM5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CEACAM5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CEACAM5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.