Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CEACAM1 Double Nickase Plasmid (m): sc-424008-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEACAM1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CEACAM1 Double Nickase Plasmid (m) and CEACAM1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ceacam1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEACAM1 Double Nickase Plasmid (m) | sc-424008-NIC | 20 µg | $410.00 | |||
CEACAM1 Double Nickase Plasmid (m2) | sc-424008-NIC-2 | 20 µg | $410.00 |
Ceacam1 encodes CEACAM1, an immunoglobulin superfamily adhesion receptor expressed on epithelial, endothelial, and multiple immune cell types in mouse. CEACAM1 participates in homophilic and heterophilic interactions that regulate cell–cell contact, tissue organization, and barrier-associated signaling, and it can modulate inhibitory signaling through ITIM-dependent pathways in leukocytes. Through effects on adhesion dynamics, cytoskeletal remodeling, and receptor crosstalk, CEACAM1 influences processes such as angiogenesis, inflammatory responses, and epithelial homeostasis. Dysregulated CEACAM1 activity has been associated with altered immune regulation and tumor biology in experimental models, supporting its use as a target in mechanistic studies of inflammation and cancer-relevant microenvironments.
CEACAM1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ceacam1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ceacam1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ceacam1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ceacam1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.