Cdx1 Double Nickase Plasmid (h): sc-402522-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdx1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdx1 Double Nickase Plasmid (h) and Cdx1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdx1 Antibody (D-4): sc-515146
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdx1 Double Nickase Plasmid (h) | sc-402522-NIC | 20 µg | $410.00 | |||
Cdx1 Double Nickase Plasmid (h2) | sc-402522-NIC-2 | 20 µg | $410.00 |
CDX1 encodes the caudal type homeobox transcription factor Cdx1, a sequence-specific DNA-binding regulator that contributes to anterior–posterior patterning and intestinal epithelial differentiation. Cdx1 integrates developmental signaling inputs, including Wnt/β-catenin and retinoic acid–responsive programs, to shape lineage-specific transcriptional networks and regional identity. In adult tissues, CDX1 helps maintain epithelial homeostasis and influences proliferation–differentiation balance through transcriptional control of downstream target genes. Dysregulated CDX1 expression and altered CDX family activity have been associated with intestinal metaplasia and colorectal tumor biology, making it a useful locus for mechanistic studies of epithelial transformation and differentiation.
Cdx1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.