CUTL1/CUX1/CDP Antibody (B-10): sc-514008

CDP Antibody (B-10) is a mouse monoclonal IgG3 kappa light chain antibody designed to recognize the CCAAT displacement protein (CDP), also known as CUX1 or CUTL1, with an epitope mapping between amino acids 1308-1332 at the C-terminus of the mouse CDP protein. CDP monoclonal antibody (B-10) exhibits broad species reactivity, effectively detecting CDP from mouse, rat, and human origins through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and multiple conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-CDP antibody (B-10) provides versatile options for researchers. CDP, also called ASP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, and GOLIM6, is a crucial homeodomain-containing transcriptional repressor located predominantly in the nucleus, where CDP regulates the transcription of developmentally controlled genes by competing with transcriptional activators for binding to promoter regions. This localization is essential for CDP′s role in modulating gene expression and maintaining proper cellular function. CDP contains three cut repeats that serve as DNA binding domains, enabling CDP to interact with DNA both independently and in conjunction with its homeodomain. Additionally, CDP is a key component of the HiNF-D complex, which includes cyclin A, Cdc2, and an Rb-related protein. This complex is vital for the regulation of histone gene transcription, specifically histones H4, H3, and H1, which are necessary for chromatin assembly and the progression of cells from the G1 phase to the S phase of the cell cycle. By controlling histone expression, CDP plays an integral role in ensuring genomic integrity and facilitating proper cell division. CDP monoclonal antibody (B-10)′s ability to reliably detect CDP across multiple species and experimental platforms makes this product an indispensable tool for researchers studying gene regulation, cell cycle dynamics, and the molecular mechanisms underlying development and various diseases.

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CUTL1/CUX1/CDP Antibody (B-10) References:

1. Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut.  |  Neufeld, EJ., et al. 1992. Nat Genet. 1: 50-5. PMID: 1301999
2. Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut.  |  Seto, H., et al. 2006. Genes Cells. 11: 499-512. PMID: 16629902
3. Differentiation-induced cleavage of Cutl1/CDP generates a novel dominant-negative isoform that regulates mammary gene expression.  |  Maitra, U., et al. 2006. Mol Cell Biol. 26: 7466-78. PMID: 17015474
4. Transgenic mice expressing the p75 CCAAT-displacement protein/Cut homeobox isoform develop a myeloproliferative disease-like myeloid leukemia.  |  Cadieux, C., et al. 2006. Cancer Res. 66: 9492-501. PMID: 17018605
5. Conserved cut repeats in the human cut homeodomain protein function as DNA binding domains.  |  Harada, R., et al. 1994. J Biol Chem. 269: 2062-7. PMID: 7904999
6. The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype.  |  Valarché, I., et al. 1993. Development. 119: 881-96. PMID: 7910552
7. CCAAT displacement protein competes with multiple transcriptional activators for binding to four sites in the proximal gp91phox promoter.  |  Luo, W. and Skalnik, DG. 1996. J Biol Chem. 271: 18203-10. PMID: 8663528
8. CDP/cut is the DNA-binding subunit of histone gene transcription factor HiNF-D: a mechanism for gene regulation at the G1/S phase cell cycle transition point independent of transcription factor E2F.  |  van Wijnen, AJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 11516-21. PMID: 8876167

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUTL1/CUX1/CDP Antibody (B-10) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-CUTL1/CUX1/CDP Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUTL1/CUX1/CDP Antibody (B-10) Specifications

Alternate Product Name: CDP

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1308-1332

Epitope Target: CDP

Epitope Species: mouse

Product Reactivity/Detection: CDP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for CUTL1/CUX1/CDP Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent