Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdk9 Antibody (D-7): sc-13130. Western blot analysis of Cdk9 expression in HeLa (A) and KNRK (B) nuclear extracts and HeLa (C), L8 (D), HL-60 (E) and NIH/3T3 (F) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdk9 Double Nickase Plasmid (m): sc-430774-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdk9 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdk9 Double Nickase Plasmid (m) and Cdk9 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cdk9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk9 Antibody (D-7): sc-13130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdk9 Double Nickase Plasmid (m) | sc-430774-NIC | 20 µg | $410.00 |