Cdk2 Antibody (0.N.198) recommended for detection of Cdk2 of mouse, rat and human origin by WB and IP

Cdk2 Antibody (0.N.198): sc-70829

Cdk2 Antibody (0.N.198) is rated 4.0 out of 5 by 4.
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Datasheets

    • Cdk2 Antibody (0.N.198) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 200 µg/ml
    • raised against human recombinant Cdk2
    • recommended for detection of Cdk2 of mouse, rat and human origin by WB and IP
    • See Cdk2 (D-12): sc-6248 for Cdk2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

CDK2的稀释倍数是多少啊

Asked by: 奔跑的小龙
感谢您的提问。免疫印迹 (起始稀释度 1:200, 稀释范围 1:100-1:1000) ,免疫沉淀[100-500 µg 总蛋白 (1 ml 细胞裂解液) 中加1-2 µg抗体]. Thank you for your question. Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)]
Answered by: Technical Support 8
Date published: 2018-12-21

How much sample do you recommend loading on the gel for Western blot detection using Cdk2 (0.N.198): sc-70829?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Clean The western blot effect is good antibody, is very clean, we are very satisfied.
Date published: 2017-04-10
Rated 4 out of 5 by from Good WB publishable data gathered with human cells Good WBand IF publishable data gathered with 3T3 cells and F9 cells
Date published: 2017-03-28
Rated 4 out of 5 by from Good WB publishable data gathered with human cells Good WB publishable data gathered with human cells. -SCBT Publication Review
Date published: 2015-04-13
Rated 3 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2013-10-30
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