CdGAP Antibody (G-8): sc-393839

CdGAP Antibody (G-8) is a mouse monoclonal IgM kappa antibody that detects CdGAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CdGAP antibody (G-8) is available as non-conjugated format. CdGAP protein, also known as Cdc42 GTPase-activating protein, functions as a crucial regulator within the superfamily of GTP-binding proteins, including Ras proteins. This 1,444 amino acid protein acts primarily as a GTPase-activating protein for Rho GTPases Cdc42 and RAC1, without interacting with RhoA. CdGAP mediates various signaling pathways influencing transcriptional activation, cell cycle control, and actin reorganization, all vital for proper cellular function and response to external stimuli. CdGAP overexpression in Cos-7 cells induces membrane blebbing, suggesting potential involvement in apoptosis. Human CdGAP undergoes phosphorylation at threonine 776 through interactions with glycogen synthase kinase 3 alpha and beta, potentially modulating activity and function. CdGAP shows ubiquitous expression across tissues, with notably high levels in muscle and heart, highlighting significance in various physiological processes.

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CdGAP Antibody (G-8) References:

1. The activity of the GTPase-activating protein CdGAP is regulated by the endocytic protein intersectin.  |  Jenna, S., et al. 2002. J Biol Chem. 277: 6366-73. PMID: 11744688
2. Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells.  |  Itoh, RE., et al. 2002. Mol Cell Biol. 22: 6582-91. PMID: 12192056
3. Extracellular signal-regulated kinase 1 interacts with and phosphorylates CdGAP at an important regulatory site.  |  Tcherkezian, J., et al. 2005. Mol Cell Biol. 25: 6314-29. PMID: 16024771
4. The human orthologue of CdGAP is a phosphoprotein and a GTPase-activating protein for Cdc42 and Rac1 but not RhoA.  |  Tcherkezian, J., et al. 2006. Biol Cell. 98: 445-56. PMID: 16519628
5. CdGAP associates with actopaxin to regulate integrin-dependent changes in cell morphology and motility.  |  LaLonde, DP., et al. 2006. Curr Biol. 16: 1375-85. PMID: 16860736
6. Glycogen synthase kinase-3 phosphorylates CdGAP at a consensus ERK 1 regulatory site.  |  Danek, EI., et al. 2007. J Biol Chem. 282: 3624-31. PMID: 17158447
7. Differential gene expression analysis of tubule forming and non-tubule forming endothelial cells: CDC42GAP as a counter-regulator in tubule formation.  |  Engelse, MA., et al. 2008. Angiogenesis. 11: 153-67. PMID: 18060510
8. CdGAP/ARHGAP31, a Cdc42/Rac1 GTPase regulator, is critical for vascular development and VEGF-mediated angiogenesis.  |  Caron, C., et al. 2016. Sci Rep. 6: 27485. PMID: 27270835
9. CdGAP promotes prostate cancer metastasis by regulating epithelial-to-mesenchymal transition, cell cycle progression, and apoptosis.  |  Mehra, C., et al. 2021. Commun Biol. 4: 1042. PMID: 34493786
10. CdGAP maintains podocyte function and modulates focal adhesions in a Src kinase-dependent manner.  |  Matsuda, J., et al. 2022. Sci Rep. 12: 18657. PMID: 36333327
11. CdGAP is a talin-binding protein and a target of TGF-β signaling that promotes HER2-positive breast cancer growth and metastasis.  |  He, Y., et al. 2023. Cell Rep. 42: 112936. PMID: 37552602
12. CdGAP, a novel proline-rich GTPase-activating protein for Cdc42 and Rac.  |  Lamarche-Vane, N. and Hall, A. 1998. J Biol Chem. 273: 29172-7. PMID: 9786927

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CdGAP Antibody (G-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CdGAP Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CdGAP Antibody (G-8) Specifications

Alternate Product Name: CdGAP

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1369-1392

Epitope Target: CdGAP

Epitope Species: human

Product Reactivity/Detection: CdGAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CdGAP Antibody (G-8): P: Blocking Peptide