Cdc5L Double Nickase Plasmid (h): sc-404611-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc5L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc5L Double Nickase Plasmid (h) and Cdc5L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC5L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc5L Antibody (D-11): sc-398280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc5L Double Nickase Plasmid (h) | sc-404611-NIC | 20 µg | $410.00 |
CDC5L encodes Cdc5L, a conserved nuclear protein that functions as a core component of the Prp19-associated complex required for spliceosome activation and pre-mRNA splicing. Cdc5L supports correct exon–intron processing and coordinates with cell cycle control by promoting mitotic progression, linking RNA metabolism to checkpoint and DNA damage response pathways. Through these roles, CDC5L influences transcriptome fidelity and genome stability, processes frequently perturbed in proliferative disorders and cancers. Altered CDC5L activity has been associated with dysregulated splicing programs and aberrant cell cycle dynamics, making it a useful target for mechanistic studies of RNA processing–driven phenotypes.
Cdc5L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC5L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC5L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC5L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC5L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.