Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc25C Double Nickase Plasmid (h): sc-400833-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc25C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc25C Double Nickase Plasmid (h) and Cdc25C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC25C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc25C Antibody (H-6): sc-13138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25C Double Nickase Plasmid (h) | sc-400833-NIC | 20 µg | $410.00 | |||
Cdc25C Double Nickase Plasmid (h2) | sc-400833-NIC-2 | 20 µg | $410.00 |
CDC25C encodes Cdc25C, a dual-specificity phosphatase that activates the CDK1–cyclin B complex by removing inhibitory phosphates, promoting the G2/M transition and entry into mitosis. Its activity is tightly controlled by checkpoint signaling, including ATR/CHK1 and p53-linked pathways, which restrain Cdc25C during DNA damage and replication stress to maintain genome integrity. Altered CDC25C regulation can disrupt mitotic timing and contribute to chromosomal instability, making it relevant to studies of oncogenic cell-cycle deregulation. Cdc25C also interfaces with 14-3-3 sequestration and phosphorylation-dependent localization, providing mechanistic entry points for dissecting checkpoint adaptation and mitotic control.
Cdc25C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC25C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC25C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC25C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC25C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.