Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD98 Double Nickase Plasmid (m): sc-421615-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD98 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD98 Double Nickase Plasmid (m) and CD98 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc3a2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD98 Antibody (F-2): sc-390154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD98 Double Nickase Plasmid (m) | sc-421615-NIC | 20 µg | $410.00 | |||
CD98 Double Nickase Plasmid (m2) | sc-421615-NIC-2 | 20 µg | $410.00 |
Slc3a2 encodes CD98 (4F2hc), a type II membrane glycoprotein that heterodimerizes with SLC7 family light chains to support neutral and branched-chain amino acid transport and metabolic homeostasis. Beyond transporter assembly and trafficking, CD98 regulates integrin-dependent adhesion and outside-in signaling, influencing cytoskeletal organization, cell migration, and survival pathways such as FAK/PI3K/AKT and MAPK. In mouse systems, Slc3a2 activity links nutrient sensing to proliferative and stress-response programs, making it relevant to studies of immune cell activation, epithelial barrier biology, and tissue remodeling. Dysregulated CD98-associated transport and integrin signaling are frequently examined in contexts of inflammatory pathology and oncogenic transformation without implying clinical outcomes.
CD98 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc3a2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc3a2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc3a2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc3a2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.