CD97 Double Nickase Plasmid (h): sc-404492-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD97 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD97 Double Nickase Plasmid (h) and CD97 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADGRE5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD97 Antibody (G-8): sc-166852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD97 Double Nickase Plasmid (h) | sc-404492-NIC | 20 µg | $410.00 | |||
CD97 Double Nickase Plasmid (h2) | sc-404492-NIC-2 | 20 µg | $410.00 |
ADGRE5 encodes CD97, an adhesion G protein–coupled receptor prominently expressed on leukocytes and implicated in cell–cell and cell–matrix interactions within immune and stromal compartments. CD97 engages ligands such as CD55/DAF and chondroitin sulfate, coordinating adhesion, migration, and activation programs that intersect with inflammatory signaling and cytoskeletal remodeling. Through GPCR-linked signaling and receptor–ligand dynamics at the cell surface, CD97 can influence leukocyte trafficking, tissue infiltration, and immune surveillance. Dysregulated CD97 expression or activity has been associated with inflammatory pathology and tumor microenvironment biology, supporting its relevance for mechanistic studies of immune regulation and disease-associated cell behavior.
CD97 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADGRE5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADGRE5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADGRE5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADGRE5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.