CD83 Antibody (4B5): sc-53855
- CD83 Antibody (4B5) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against the extracellular domain of CD83 of human origin
- recommended for detection of CD83 of human origin by WB, IP, IF and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See CD83 (D-3): sc-55536 for CD83 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD83 Antibody (4B5). This work is in progress.
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CD83 Antibody (4B5) is a rat monoclonal IgG2a antibody that detects CD83 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD83 is a heavily glycosylated membrane protein belonging to the immunoglobulin superfamily, primarily expressed in mature dendritic cells, Langerhans cells, and interdigitating reticulum cells within lymphoid tissues. The structure of CD83 features an extracellular V-type immunoglobulin-like domain, a single transmembrane domain, and a 40-residue cytoplasmic tail, which collectively facilitate CD83′s role in immune responses. CD83 serves as a marker for mature antigen-presenting dendritic cells that are essential for generating tumor-specific T cell immunity, making CD83 a critical component in the development of anti-cancer vaccines. Studies involving CD83-IgG1(fc) chimeras have revealed that CD83 functions as a sialic acid-binding immunoglobulin-like lectin (Siglec) adhesion molecule, playing a vital role in cell adhesion and signaling by mediating interactions between dendritic cells, monocytes, and CD8+ T cells. CD83′s ability to influence these interactions underscores CD83′s importance in orchestrating immune responses and highlights CD83′s potential as a therapeutic target in cancer immunotherapy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD83 Antibody (4B5) References:
- Soluble CD83 ameliorates experimental colitis in mice. | Eckhardt, J., et al. 2014. Mucosal Immunol. 7: 1006-18. PMID: 24424524
- CD83 Modulates B Cell Activation and Germinal Center Responses. | Krzyzak, L., et al. 2016. J Immunol. 196: 3581-94. PMID: 26983787
- Human CD83-targeted chimeric antigen receptor T cells prevent and treat graft-versus-host disease. | Shrestha, B., et al. 2020. J Clin Invest. 130: 4652-4662. PMID: 32437331
- CD83, a Novel MAPK Signaling Pathway Interactor, Determines Ovarian Cancer Cell Fate. | Batool, A., et al. 2020. Cancers (Basel). 12: PMID: 32823589
- Influenza Virus Neuraminidase Engages CD83 and Promotes Pulmonary Injury. | Ma, N., et al. 2021. J Virol. 95: PMID: 33177200
- CD83 expression regulates antibody production in response to influenza A virus infection. | Akauliya, M., et al. 2020. Virol J. 17: 194. PMID: 33302987
- Soluble CD83 improves and accelerates wound healing by the induction of pro-resolving macrophages. | Royzman, D., et al. 2022. Front Immunol. 13: 1012647. PMID: 36248909
- CD83 acts as immediate early response gene in activated macrophages and exhibits specific intracellular trafficking properties. | Langguth, P., et al. 2023. Biochem Biophys Res Commun. 647: 37-46. PMID: 36709671
- MCAM+CD161- Th17 Subset Expressing CD83 Enhances Tc17 Response in Psoriasis. | Maeda, K., et al. 2023. J Immunol. 210: 1867-1881. PMID: 37186262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD83 Antibody (4B5) | sc-53855 | 200 µg/ml | $322.00 | |||
CD83 Antibody (4B5) FITC | sc-53855 FITC | 200 µg/ml | $336.00 | |||
CD83 Antibody (4B5) PE | sc-53855 PE | 200 µg/ml | $349.00 |