CD73 Antibody (IE9): sc-32299

CD73 (IE9) is a mouse monoclonal IgG3 kappa light chain antibody that detects CD73 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-CD73 antibody (IE9) is available in non-conjugated form. CD73 (also designated 5′-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 exists as a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Additionally, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

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CD73 Antibody (IE9) References:

1. Regulation of ecto-5'-nucleotidase by TNF-alpha in human endothelial cells.  |  Kalsi, K., et al. 2002. Mol Cell Biochem. 232: 113-9. PMID: 12030367
2. Adherent leukocytes prevent adenosine formation and impair endothelial barrier function by Ecto-5'-nucleotidase/CD73-dependent mechanism.  |  Henttinen, T., et al. 2003. J Biol Chem. 278: 24888-95. PMID: 12707258
3. IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5'-nucleotidase) up-regulation.  |  Niemelä, J., et al. 2004. J Immunol. 172: 1646-53. PMID: 14734746
4. Ecto-5'-nucleotidase (CD73)-mediated adenosine production is tissue protective in a model of bleomycin-induced lung injury.  |  Volmer, JB., et al. 2006. J Immunol. 176: 4449-58. PMID: 16547283
5. Expression of CD73 and its ecto-5'-nucleotidase activity are elevated in papillary thyroid carcinomas.  |  Kondo, T., et al. 2006. Histopathology. 48: 612-4. PMID: 16623792
6. CD73/ecto-5'-nucleotidase protects against vascular inflammation and neointima formation.  |  Zernecke, A., et al. 2006. Circulation. 113: 2120-7. PMID: 16636171
7. Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts.  |  Muñoz-Fernández, R., et al. 2006. J Immunol. 177: 280-9. PMID: 16785523
8. ATP-consuming and ATP-generating enzymes secreted by pancreas.  |  Yegutkin, GG., et al. 2006. J Biol Chem. 281: 29441-7. PMID: 16885159
9. CD73 Inhibits cGAS-STING and Cooperates with CD39 to Promote Pancreatic Cancer.  |  Jacoberger-Foissac, C., et al. 2023. Cancer Immunol Res. 11: 56-71. PMID: 36409930
10. Proteolytic regulation of CD73 by TRIM21 orchestrates tumor immunogenicity.  |  Fu, Z., et al. 2023. Sci Adv. 9: eadd6626. PMID: 36608132
11. CD39/CD73/A2AR pathway and cancer immunotherapy.  |  Xia, C., et al. 2023. Mol Cancer. 22: 44. PMID: 36859386
12. CD73 expression and fyn-dependent signaling on murine lymphocytes.  |  Yamashita, Y., et al. 1998. Eur J Immunol. 28: 2981-90. PMID: 9808167

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD73 Antibody (IE9) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-CD73 Antibody (IE9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD73 Antibody (IE9) Specifications

Alternate Product Name: 5'-nucleotidase

Product Clone Name: IE9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids:

Epitope Target: CD73

Epitope Species: human

Product Reactivity/Detection: CD73

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for CD73 Antibody (IE9): N/A

The preferred CD73 antibody is CD73 Antibody (2B6): sc-130006.