CD45RC Antibody (OX22): sc-53049
- CD45RC Antibody (OX22) is a mouse monoclonal IgG1 κ CD45RC antibody provided at 200 µg/ml
- raised against stimulated lymphocytes of rat origin
- CD45RC Antibody (OX22) is recommended for detection of CD45RC of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-CD45RC Antibody (OX22) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45RC Antibody (OX22). This work is in progress.
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CD45RC Antibody (OX22) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD45RC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD45RC antibody (OX22) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD45RC, also known as CD45 or PTPRC, is a transmembrane glycoprotein that plays a crucial role in the immune system, being broadly expressed among hematopoietic cells. CD45RC features multiple isoforms generated through alternative splicing of exons 4, 5, and 6, which determine specific interactions and signaling pathways activated in different immune cell types. The extracellular domain variations allow for differential recognition by specific monoclonal antibodies, while the conserved intracellular domain maintains the phosphotyrosine phosphatase activity necessary for modulating T cell receptor signaling. This activity is vital for the regulation of tyrosine phosphorylation, which is critical for T cell activation and immune response. CD45RC interacts with Src and Syk family protein tyrosine kinases, including Lck, Fyn, and ZAP-70, which are essential for initiating signaling cascades upon T cell receptor engagement. The ability of CD45RC to modulate these pathways underscores its importance in maintaining immune homeostasis and response.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD45RC Antibody (OX22) References:
- Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. | Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
- Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. | Johnson, P., et al. 1989. J Exp Med. 169: 1179-84. PMID: 2466938
- Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. | Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
- Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. | Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
- The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections. | West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
- New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections. | Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
- Regulation of immune function by protein tyrosine phosphatases. | Okumura, M. and Thomas, ML. 1995. Curr Opin Immunol. 7: 312-9. PMID: 7546394
- Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. | Trowbridge, IS. 1978. J Exp Med. 148: 313-23. PMID: 78961
- CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. | Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45RC Antibody (OX22) | sc-53049 | 200 µg/ml | $322.00 | |||
CD45RC Antibody (OX22) AC | sc-53049 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD45RC Antibody (OX22) HRP | sc-53049 HRP | 200 µg/ml | $322.00 | |||
CD45RC Antibody (OX22) FITC | sc-53049 FITC | 200 µg/ml | $336.00 | |||
CD45RC Antibody (OX22) PE | sc-53049 PE | 200 µg/ml | $349.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 488 | sc-53049 AF488 | 200 µg/ml | $364.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 546 | sc-53049 AF546 | 200 µg/ml | $364.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 594 | sc-53049 AF594 | 200 µg/ml | $364.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 647 | sc-53049 AF647 | 200 µg/ml | $364.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 680 | sc-53049 AF680 | 200 µg/ml | $364.00 | |||
CD45RC Antibody (OX22) Alexa Fluor® 790 | sc-53049 AF790 | 200 µg/ml | $364.00 |