CD42b Antibody (E-8): sc-271171

CD42b Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD42b protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD42b (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. CD42b protein, also known as glycoprotein Ib α (GPIb α), plays a crucial role in hemostasis as CD42b forms a noncovalent complex with CD42a, functioning as a receptor for von Willebrand′s factor. This interaction is vital for platelet adhesion to the vascular endothelium at sites of injury, thereby facilitating the formation of a platelet plug and preventing excessive bleeding. CD42b expression on the surface of platelets and megakaryocytes underscores CD42b′s importance in the regulation of platelet function and the overall coagulation process. Understanding CD42b dynamics and interactions can provide insights into various bleeding disorders and potential therapeutic targets for enhancing hemostatic function.

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CD42b Antibody (E-8) References:

1. High-resolution CryoFESEM of individual cell adhesion molecules (CAMs) in the glycocalyx of human platelets: detection of P-selectin (CD62P), GPI-IX complex (CD42A/CD42B alpha,B beta), and integrin GPIIbIIIa (CD41/CD61) by immunogold labeling and stereo imaging.  |  Erlandsen, SL., et al. 2001. J Histochem Cytochem. 49: 809-19. PMID: 11410606
2. The death of human platelets during incubation in citrated plasma involves shedding of CD42b and aggregation of dead platelets.  |  Hartley, PS., et al. 2006. Thromb Haemost. 95: 100-6. PMID: 16543968
3. Histamine reduces GPIbα-mediated adhesion of platelets to TNF-α-activated vascular endothelium.  |  Brown, TP., et al. 2013. Thromb Res. 131: 150-7. PMID: 23260446
4. Increased GPIbα shedding from platelets treated with immune thrombocytopenia plasma.  |  Wei, G., et al. 2019. Int Immunopharmacol. 66: 91-98. PMID: 30445311
5. The Direct Thrombin Inhibitors Dabigatran and Lepirudin Inhibit GPIbα-Mediated Platelet Aggregation.  |  Trabold, K., et al. 2019. Thromb Haemost. 119: 916-929. PMID: 31005062
6. Appropriation of GPIbα from platelet-derived extracellular vesicles supports monocyte recruitment in systemic inflammation.  |  Chimen, M., et al. 2020. Haematologica. 105: 1248-1261. PMID: 31467123
7. Platelet surface GPIbα, activated GPIIb-IIIa, and P-selectin levels in adult veno-arterial extracorporeal membrane oxygenation patients.  |  Mazzeffi, M., et al. 2022. Platelets. 33: 116-122. PMID: 33284729
8. Detection of anti-GPIbα autoantibodies in a case of immune thrombocytopenia following COVID-19 vaccination.  |  Nakamura, T., et al. 2022. Thromb Res. 209: 80-83. PMID: 34894532
9. Fast clearance of platelets in a commonly used mouse model for GPIbα is impeded by an anti-GPIbβ antibody derivative.  |  Chen, W., et al. 2022. J Thromb Haemost. 20: 1451-1463. PMID: 35305057
10. GPIbα shedding in platelets is controlled by strict intracellular containment of both enzyme and substrate.  |  Six, KR., et al. 2023. J Thromb Haemost. 21: 2223-2235. PMID: 37001816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD42b Antibody (E-8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CD42b Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD42b Antibody (E-8) Specifications

Alternate Product Name: GP1BA

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 587-620

Epitope Target: CD42b

Epitope Species: human

Product Reactivity/Detection: CD42b α chain

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CD42b Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred CD42b antibody is CD42b Antibody (SZ2): sc-59052.