CD33 (h): 293T Lysate: sc-114798

CD33 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, enhancing its transfection efficiency and protein expression capabilities. This lysate is specifically enriched with human CD33, a transmembrane receptor expressed on myeloid cells, including monocytes, macrophages, and dendritic cells. CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family and plays a key role in cell-cell interactions, signaling, and modulation of immune responses. In research, CD33 (h): 293T Lysate is used to study the molecular mechanisms of CD33-mediated signaling pathways and its function in the immune system. Researchers employ this lysate in Western blotting to detect and quantify CD33 expression levels under different experimental conditions, providing insights into its regulation and activation. The lysate is also utilized in co-immunoprecipitation assays to identify protein-protein interactions involving CD33, helping to explain its role in immune cell signaling networks. Additionally, CD33 (h): 293T Lysate is valuable in flow cytometry and binding studies to explore the interactions of CD33 with its ligands and antibodies, contributing to the understanding of its role in immune cell communication and modulation. Functional assays using this lysate can assess the impact of CD33 signaling on cellular responses, such as cytokine production and phagocytosis.