CD3 Antibody (KT3): sc-20080

CD3 Antibody (KT3) is a rat monoclonal IgG2a antibody designed to recognize mouse CD3, exhibiting high reactivity in flow cytometry (FCM) applications. CD3, also known as the T cell co-receptor complex or TCR-CD3 complex, is a crucial component of the T cell receptor (TCR) signaling machinery located on the surface of T lymphocytes. Structurally, the CD3 complex comprises five invariant polypeptide chains, gamma, epsilon, delta, zeta, and eta, that assemble into three distinct dimers: γε, δε, and ζζ or ζη. This intricate structure is essential for transducing extracellular antigen recognition into intracellular activation signals, thereby facilitating T cell activation, proliferation, and differentiation. CD3 interacts with several key proteins, including the tyrosine kinases ZAP-70 and Syk, which bind to phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3 chains, initiating downstream signaling cascades vital for immune responses. Additionally, CD3 interacts with proteins such as Lck and Fyn, which are involved in the initial phosphorylation events following TCR engagement, further propagating the activation signal. These interactions are fundamental for the proper transmission of activation signals that regulate immune functions and maintain immune homeostasis. By targeting the CD3 complex, anti-CD3 antibody (KT3) enables researchers to investigate T cell activation, signal transduction pathways, and immune response mechanisms. The ability to accurately detect CD3 expression on mouse cells makes CD3 monoclonal antibody (KT3) an indispensable tool for immunological research, disease modeling, and therapeutic development.

The preferred CD3 antibody is CD3 Antibody (PC3/188A): sc-20047.