CD2BP2 Double Nickase Plasmid (m): sc-427646-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD2BP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD2BP2 Double Nickase Plasmid (m) and CD2BP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd2bp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD2BP2 Double Nickase Plasmid (m) | sc-427646-NIC | 20 µg | $410.00 |
Mouse Cd2bp2 encodes CD2BP2, an adaptor protein implicated in pre-mRNA splicing through interactions with core spliceosomal components and regulation of splice site selection. CD2BP2 has been linked to nuclear speckle organization and coupling of transcription with RNA processing, influencing proteome diversity via alternative splicing programs. Through these RNA maturation pathways, Cd2bp2 can modulate cell-state transitions such as differentiation and stress responses that depend on precise transcript isoform balance. Dysregulated splicing is a common feature in cancer and neurodevelopmental and neurodegenerative disorders, making Cd2bp2 a useful node for mechanistic studies of RNA-processing-associated phenotypes in mouse models.
CD2BP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd2bp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd2bp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd2bp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd2bp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.