CD28 Antibody (YTH 913.12): sc-59004

CD28 Antibody (YTH 913.12) is a rat monoclonal IgG2b antibody that detects CD28 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD28 plays a crucial role in T cell activation and proliferation by serving as a primary co-stimulatory receptor on T cells, which is essential for effective immune responses. Upon engagement with CD80 and CD86 ligands expressed on antigen-presenting cells, CD28 enhances T cell receptor signaling, leading to increased production of cytokines and promoting T cell survival. This interaction is vital for the initiation of adaptive immunity, as CD28 not only amplifies the T cell response but also helps in the formation of immunological memory. Furthermore, CD28 expression is tightly regulated, with higher levels on resting T cells and decreasing upon activation, which underscores CD28′s importance in maintaining immune homeostasis. Anti-CD28 antibody (YTH 913.12) is an invaluable tool for researchers studying T cell biology and the mechanisms of immune regulation.

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CD28 Antibody (YTH 913.12) References:

1. CD28 signals in the immature immunological synapse.  |  Andres, PG., et al. 2004. J Immunol. 172: 5880-6. PMID: 15128767
2. New insights in CD28-independent allograft rejection.  |  Habicht, A., et al. 2007. Am J Transplant. 7: 1917-26. PMID: 17617854
3. CD28 co-signaling in the adaptive immune response.  |  Riha, P. and Rudd, CE. 2010. Self Nonself. 1: 231-240. PMID: 21487479
4. B7-h2 is a costimulatory ligand for CD28 in human.  |  Yao, S., et al. 2011. Immunity. 34: 729-40. PMID: 21530327
5. Enhancement of soluble CD28 levels in the serum of Graves' disease.  |  Sun, Z., et al. 2014. Cent Eur J Immunol. 39: 216-22. PMID: 26155127
6. Selective blockade of CD28 on human T cells facilitates regulation of alloimmune responses.  |  Zaitsu, M., et al. 2017. JCI Insight. 2: PMID: 28978798
7. Regulatory T cell subsets are differentially dependent on CD28 for their proliferation.  |  Wakamatsu, E., et al. 2018. Mol Immunol. 101: 92-101. PMID: 29909367
8. SAP interacts with CD28 to inhibit PD-1 signaling in T lymphocytes.  |  Sandigursky, S., et al. 2020. Clin Immunol. 217: 108485. PMID: 32504780
9. The role of CD28 in the prognosis of young lung adenocarcinoma patients.  |  Sun, D., et al. 2020. BMC Cancer. 20: 910. PMID: 32967633
10. IL-7 Promotes the Expansion of Circulating CD28- Cytotoxic T Lymphocytes in Patients With IgG4-Related Disease via the JAK Signaling.  |  Xia, CS., et al. 2022. Front Immunol. 13: 922307. PMID: 35874706

The preferred CD28 antibody is CD28 Antibody (CD28.2): sc-19655.